CHARACTERIZATION OF A MONOCLONAL-ANTIBODY PREPARED AGAINST PLANT ACTIN

被引：33

作者：

ANDERSLAND, JM

FISHER, DD

WYMER, CL

CYR, RJ

PARTHASARATHY, MV

机构：

[1] CORNELL UNIV,PLANT BIOL SECT,ITHACA,NY 14853

[2] SO REG RES CTR,NEW ORLEANS,LA

[3] PENN STATE UNIV,DEPT BIOL,UNIVERSITY PK,PA 16802

来源：

CELL MOTILITY AND THE CYTOSKELETON | 1994年 / 29卷 / 04期

关键词：

MICROFILAMENT; PHALLOIDIN; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY;

D O I：

10.1002/cm.970290406

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Anti-actin monoclonal antibodies were prepared using phalloidin-stabilized actin that was purified from pea roots by DNase I affinity chromatography. One monoclonal antibody, designated mAb3H11, bound plant actin in preliminary screenings and was further analyzed. Immunoblot analysis showed that this antibody had a high affinity for plant actin in crude and purified preparations but a low affinity for rabbit muscle actin. In immunoblots of plant extracts separated on two-dimensional gels it appeared to bind all actin isoforms recognized by the JLA20 anti-chicken actin antibody. Using immunofluorescent cytochemistry, the antibody was used to observe actin filaments in aldehyde-fixed and methanol-treated tobacco protoplasts. These results indicate that mAb3H11 should be a useful reagent for the study of plant actins. (C) 1994 Wiley-Liss, Inc.

引用

页码：339 / 344

页数：6

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