ISOLATION AND IDENTIFICATION OF TRANS-4-(METHYLTHIO)-3-BUTENYL GLUCOSINOLATE FROM RADISH ROOTS (RAPHANUS-SATIVUS L)

A procedure for preparative isolation of trans-4-(methylthio)-3-butenyl glucosinolate (4MTB-GLS) as a potassium salt from radish roots is described. The main steps of this procedure are (i) lyophilization of suitable ripe radish roots; (ii) extraction using 70% methanol with 50 mM imidazole-formate, pH 4.15; (iii) ion-exchange chromatography on DEAE-Sephadex A-25; (iv) concentration and desalting; and (v) precipitation of 4MTB-GLS in ethanol at -20-degrees-C. The 4MTB-GLS was identified on the basis of its physicochemical properties, UV, IR, H-1 and C-13 NMR, and mass spectra.