DEVELOPMENT OF A NEW PROLIFIGEN TPA IRMA ASSAY USING MONOCLONAL ANTI-CYTOKERATIN ANTIBODIES

被引：5

作者：

SUNDSTROM, BE ^{[1
]}

DAMICO, Y ^{[1
]}

BRUNDELL, J ^{[1
]}

机构：

[1] AB SANGTEC MED,S-16102 BROMMA,SWEDEN

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 1995年 / 10卷 / 03期

关键词：

TISSUE POLYPEPTIDE ANTIGEN; TUMOR MARKER; CYTOKERATIN; MONOCLONAL ANTIBODY; IMMUNOASSAY;

D O I：

10.1177/172460089501000307

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new immunoassay for TPA determination has been developed in which anticytokeratin monoclonal antibodies are used. A three-MAb combination with different anticytokeratin antibody specificities has been selected to mimic the complex pattern found for the polyclonal anti-TPA antibody. The accuracy of the assay is good, as judged from analytical- recovery experiments, analysis of quality-assessment samples and comparison with the polyclonal Prolifigen TPA IRMA. No significant interferences or cross-reactivities have been identified. The lower limit of detection of the assay (mean + 3 SD of the zero standard) is below 15 U/L and the imprecision in low, giving a working range of 15-4000 U/L. The improved handling of the assay, including a single incubation step of 2 hours with shaking at room temperature, results in a narrower normal distribution, thereby giving a better separation of normal and pathological samples.

引用

页码：166 / 173

页数：8

共 27 条

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