STRUCTURE OF IGE EPITOPES ON A NEW 39-KD ALLERGEN MOLECULE FROM THE HOUSE-DUST MITE, DERMATOPHAGOIDES-FARINAE

被引：17
作者：
AKI, T
ONO, K
HIDAKA, Y
SHIMONISHI, Y
JYO, T
WADA, T
YAMASHITA, M
SHIGETA, S
MUROOKA, Y
OKA, S
机构：
[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIGASHIHIROSHIMA 724,JAPAN
[2] OSAKA UNIV,INST PROT RES,SUITA,OSAKA,JAPAN
[3] HIROSHIMA PREFECTURAL HOSP,DEPT INTERNAL MED 3,HIROSHIMA,JAPAN
[4] FUMAKILLA LTD,DEV & ADM SECT,HIROSHIMA,JAPAN
来源：
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1994年 / 103卷 / 04期
关键词：
IGE EPITOPE; EPITOPE MAPPING; MITE ALLERGEN; DERMATOPHAGOIDES FARINAE;
D O I：
10.1159/000236654
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Two IgE epitope sequences comprising Ser(56)-Pro-Val-Thr-Lys-Arg-Ala-Ser-Leu-Lys-Ile-Asp-Ser-Lys-Lys(70) and Asp(104)-Val-Glu-Leu-Ser-Leu-Arg-Ser-Ser-Asp-Ile-Ala(115) were identified by deletion analysis of the cDNA encoding a new 39-kD protein of mite allergen. A synthetic dodecapeptide corresponding to the latter epitope sequence functioned as a monovalent and mite-specific hapten. Replacement of each of the 12 amino acid residues with Gly, using site-directed mutagenesis, indicated that Arg(110) may play a central role in IgE binding. However, the 8 allergic sera tested exhibited a wide variation in their amino acid residues required for reactivity to IgE in allergic sera.
引用
收藏
页码：357 / 364
页数：8
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