THE EFFECTS OF OLIGOSACCHARIDE TRIMMING INHIBITORS ON GLYCOPROTEIN EXPRESSION AND INFECTIVITY OF JUNIN VIRUS

被引：8

作者：

SILBER, AM ^{[1
]}

CANDURRA, NA ^{[1
]}

DAMONTE, EB ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT QUIM BIOL,VIROL LAB,CIUDAD UNIV,PABELLON 2,RA-1428 BUENOS AIRES,ARGENTINA

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 109卷 / 01期

关键词：

OLIGOSACCHARIDE TRIMMING INHIBITORS; VIRUS INFECTIVITY; GLYCOPROTEIN EXPRESSION; GLYCOPROTEIN PROCESSING; JUNIN VIRUS;

D O I：

10.1111/j.1574-6968.1993.tb06140.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucosidases I and II, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase I and II, respectively, showed any activity against JV multiplication. These results are the first evidence that the acquisition of a complex form of the envelope glycoprotein oligosaccharide chains is not essential for JV infectivity. The effect of bromoconduritol was reversible and probably due to the formation of an unstable intermediate oligosaccharide structure which may be more sensitive to degradative proteolysis.

引用

页码：39 / 44

页数：6

共 14 条

[1] INHIBITION OF N-LINKED OLIGOSACCHARIDE TRIMMING DOES NOT INTERFERE WITH SURFACE EXPRESSION OF CERTAIN INTEGRAL MEMBRANE-PROTEINS [J].