THE DEMONSTRATION OF CELL-SURFACE ANTIGENS ON T-CELLS, B-CELLS AND ACCESSORY CELLS IN PARAFFIN-EMBEDDED HUMAN-TISSUES

被引：65

作者：

COLLINGS, LA ^{[1
]}

POULTER, LW ^{[1
]}

JANOSSY, G ^{[1
]}

机构：

[1] ROYAL FREE HOSP, SCH MED, DEPT IMMUNOL, LONDON NW3 2QG, ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1984年 / 75卷 / 02期

关键词：

D O I：

10.1016/0022-1759(84)90106-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for processing fresh tissue that allows immunohistological analysis on paraffin sections is described. The method is based on the use of periodate-lysine-paraformaldehyde fixation. The effects of variation in fixation time, concentration of paraformaldehyde, dehydration, clearing, wax embedding and enzyme treatment of cut sections were examined. An optimal processing procedure was established that retains good tissue morphology and allowed 21 out of 27 monoclonal antibodies tested to be used successfully on paraffin sections to identify all major cell subpopulations by their membrane antigenic characteristics. The value of this approach in studying the immunopathology of potentially dangerous infectious diseases and in leukemia/lymphoma diagnosis is discussed.

引用

页码：227 / 239

页数：13

共 32 条

[11] IMPROVED DOUBLE IMMUNOENZYME LABELING USING ALKALINE-PHOSPHATASE AND HORSERADISH-PEROXIDASE [J].

MALIK, NJ ;

DAYMON, ME .

JOURNAL OF CLINICAL PATHOLOGY, 1982, 35 (10) :1092-1094

[12]

Mason D., 1983, IMMUNOCYTOCHEMISTRY, P113

[13]

MASON D.Y., 1982, MONOCLONAL ANTIBODIE, P585

[14] PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE - NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY [J].

MCLEAN, IW ;

NAKANE, PK .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1974, 22 (12) :1077-1083

[15]

MCMILLAN EM, 1982, ARCH PATHOL LAB MED, V106, P9

[16]

MELPHAM BL, 1979, HISTOCHEM J, V11, P345

[17]

MONTANO L, 1982, HEPATOLOGY, V2, P557

[18]

POULTER LW, 1984, CLIN EXP IMMUNOL, V55, P611

[19]

POULTER LW, 1983, CLIN EXP IMMUNOL, V53, P513

[20] IMMUNOHISTOLOGICAL ANALYSIS OF DELAYED-TYPE HYPERSENSITIVITY IN MAN [J].

POULTER, LW ;

SEYMOUR, GJ ;

DUKE, O ;

JANOSSY, G ;

PANAYI, G .

CELLULAR IMMUNOLOGY, 1982, 74 (02) :358-369

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