INVOLVEMENT OF THE 3 INTER-ALPHA-TRYPSIN INHIBITOR (ITI) HEAVY-CHAINS IN EACH MEMBER OF THE SERUM ITI FAMILY

被引：16
作者：
HERON, A
BOURGUIGNON, J
DIARRAMEHRPOUR, M
DAUTREAUX, B
MARTIN, JP
SESBOUE, R
机构：
[1] FAC MED PHARM ROUEN, INSERM, U295, F-76803 ST ETIENNE, FRANCE
[2] ERPUR, ROUEN, FRANCE
来源：
FEBS LETTERS | 1995年 / 374卷 / 02期
关键词：
INTER-ALPHA-TRYPSIN INHIBITOR; PRE-ALPHA-TRYPSIN INHIBITOR; RECOMBINANT DNA; HYBRID PROTEIN; POLYCLONAL ANTISERUM; WESTERN BLOTTING;
D O I：
10.1016/0014-5793(95)01103-L
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Partial cDNAs coding for each of the three human inter-alpha-trypsin inhibitor (ITI) heavy chains were expressed in a bacterial plasmid system and rabbits were immunised,vith the fusion peptides obtained. Despite the strong sequence homology of these chains, the antisera turned out to be highly specific in the analysis of corresponding mRNA translation products or partially digested serum ITI. Besides classical serum ITI members, their use in Western blotting made it possible to evidence an H3-related ITI form and a low-amount Hi-related HC/bikunin component. The relative levels of ITI family members was further studied in baboon and foetal calf sera.
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页码：195 / 198
页数：4
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