H-1-NMR AND C-13-NMR CHARACTERIZATION OF THE DIGALACTOSYLMANNOPENTAOSE LIBERATED FROM LEGUME SEED GALACTOMANNAN BY BETA-MANNANASE ACTION

被引：21

作者：

DAVIS, AL ^{[1
]}

HOFFMANN, RA ^{[1
]}

RUSSELL, AL ^{[1
]}

DEBET, M ^{[1
]}

机构：

[1] UNILEVER RES,SHARNBROOK MK44 1LQ,BEDS,ENGLAND

来源：

CARBOHYDRATE RESEARCH | 1995年 / 271卷 / 01期

关键词：

LOCUST BEAN GUM; ASPERGILLUS NIGER; BETA-D-MANNANASE; GALACTOMANNAN;

D O I：

10.1016/0008-6215(95)00038-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of Locust bean gum with an Aspergillus niger beta-D-mannanase released a wide variety of galactomannan oligomers. A single heptasaccharide, digalactosylmannopentaose, was obtained from fractionation of the mixture by size exclusion chromatography. The purity and chemical composition of the sample was demonstrated using mass spectrometry, high performance anion-exchange chromatography and monosaccharide composition analysis. The primary structure of this heptasaccharide was unambiguously identified using 2D H-1 and C-13 homonuclear and heteronuclear NMR. A complete assignment of the H-1 and C-13 signals of this oligomer was achieved, producing an NMR dataset that will be of importance in the primary structure elucidation of larger and more complex galactomannan oligomers.

引用

页码：43 / 54

页数：12

共 21 条

[1] PRACTICAL ASPECTS OF TWO-DIMENSIONAL TRANSVERSE NOE SPECTROSCOPY [J].