LIQUID-LIQUID-EXTRACTION OF A RECOMBINANT PROTEIN, CYTOCHROME B(5) WITH AQUEOUS 2-PHASE SYSTEMS OF POLYETHYLENE-GLYCOL AND POTASSIUM PHOSPHATE SALTS

被引：11
|
作者：
SARMENTO, MJ [1 ]
PIRES, MJ [1 ]
CABRAL, JMS [1 ]
AIRESBARROS, MR [1 ]
机构：
[1] Univ Tecn Lisboa, ENGN BIOQUIM LAB, INST SUPER TECN, P-1000 LISBON, PORTUGAL
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 668卷 / 01期
关键词：
D O I：
10.1016/0021-9673(94)80099-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The partitioning of cytochrome b(5) in aqueous two-phase systems of polyethylene glycol (PEG) and potassium phosphate salts was investigated. Cytochrome b(5) partitioning is enhanced with decreasing polymer molecular mass and with increasing tieline length and pH. The effect of cytochrome b(5) mutation, by substitution of the glutamic acid at positions 56 and 92 of the polypeptide chain by a lysine, on protein partitioning was also studied. Partitioning of cytochrome b(5) mutants shows the same dependence on tieline length and pH, following the order cytochrome b(5) > mutant 56 > mutant 92.
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页码：117 / 120
页数：4
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