SEQUENCING AND THE ALIGNMENT OF STRUCTURAL GENES IN THE NQR OPERON ENCODING THE NA+-TRANSLOCATING NADH-QUINONE REDUCTASE FROM VIBRIO-ALGINOLYTICUS

被引：68

作者：

HAYASHI, M

HIRAI, K

UNEMOTO, T

机构：

[1] Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba, 263

来源：

FEBS LETTERS | 1995年 / 363卷 / 1-2期

关键词：

NA+ PUMP; NADH-QUINONE REDUCTASE; NUCLEOTIDE SEQUENCE; NQR OPERON; VIBRIO ALGINOLYTICUS;

D O I：

10.1016/0014-5793(95)00283-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously cloned a part of nqr operon encoding the Na+-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus [Hayashi et al., FEES Lett, 356 (1994) 330-332], From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the gamma-subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the beta-subunit (45.3 kDa) were recognized. The gene encoding the alpha-subunit was located upstream, and together with the recent report by Beattie et al. [FEES Lett, 356 (1994) 333-338], the nqr operon was found to be constructed from six consecutive structural genes, where nqr1, nqr3 and nqr6 correspond to the alpha-, gamma-, and beta-subunits, respectively, of the NQR complex.

引用

页码：75 / 77

页数：3

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