A SET OF 97 OVERLAPPING YEAST ARTIFICIAL CHROMOSOME CLONES SPANNING THE HUMAN Y-CHROMOSOME EUCHROMATIN

被引：58

作者：

JONES, MH

KHWAJA, OSA

BRIGGS, H

LAMBSON, B

DAVEY, PM

CHALMERS, J

ZHOU, CY

WALKER, EM

ZHANG, Y

TODD, C

FERGUSONSMITH, MA

AFFARA, NA

机构：

[1] UNIV CAMBRIDGE, DEPT PATHOL, HUMAN MOLEC GENET GRP, CAMBRIDGE CB2 1QP, ENGLAND

[2] UNITED MED & DENT SCH, DIV MED & MOLEC GENET, LONDON SE1 9RT, ENGLAND

来源：

GENOMICS | 1994年 / 24卷 / 02期

关键词：

D O I：

10.1006/geno.1994.1615

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Contiguous arrays of yeast artificial chromosomes (YACs) extending from proximal heterochromatic Yq into the pseudoautosomal portion of the Y chromosome and separated by a small interval at the centromere have been constructed. A total of 97 YACs have been aligned along the Y chromosome by STS content analysis using 222 sequence tagged sites (STSs) that detect 263 loci. Forty-five of the STSs used are novel, Their inclusion provides a significant improvement over previously available maps on the density of STS coverage along the Y chromosome, reducing the average spacing to 120 kb assuming a length of 30 Mb for the euchromatin. The average size of 61 YACs determined by pulsed-held gel electrophoresis analysis was at least 0.9 Mb. Minor differences noted between the ordering of STSs on this map compared with those previously reported may be attributed to inherent polymorphism between the Y chromosomes used to construct the YAC libraries. (C) 1994 Academic Press, Inc.

引用

页码：266 / 275

页数：10

共 19 条

[1] MAPPING THE TESTIS DETERMINANTS BY AN ANALYSIS OF Y-SPECIFIC SEQUENCES IN MALES WITH APPARENT XX AND XO KARYOTYPES AND FEMALES WITH XY KARYOTYPES [J].

AFFARA, NA ;

FERGUSONSMITH, MA ;

MAGENIS, RE ;

TOLMIE, JL ;

BOYD, E ;

COOKE, A ;

JAMIESON, D ;

KWOK, K ;

MITCHELL, M ;

SNADDEN, L .

NUCLEIC ACIDS RESEARCH, 1987, 15 (18) :7325-7342

[2]

AFFARA NA, 1993, MOL GENETICS SEX DET

[3] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].

ALBERTSEN, HM ;

ABDERRAHIM, H ;

CANN, HM ;

DAUSSET, J ;

LEPASLIER, D ;

COHEN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4256-4260

[4] THE DEVELOPMENT AND APPLICATION OF AUTOMATED GRIDDING FOR EFFICIENT SCREENING OF YEAST AND BACTERIAL ORDERED LIBRARIES [J].

BENTLEY, DR ;

TODD, C ;

COLLINS, J ;

HOLLAND, J ;

DUNHAM, I ;

HASSOCK, S ;

BANKIER, A ;

GIANNELLI, F .

GENOMICS, 1992, 12 (03) :534-541

[5]

BURKE DT, 1991, METHOD ENZYMOL, V194, P251

[6] CONTINUUM OF OVERLAPPING CLONES SPANNING THE ENTIRE HUMAN CHROMOSOME-21Q [J].

CHUMAKOV, I ;

RIGAULT, P ;

GUILLOU, S ;

OUGEN, P ;

BILLAUT, A ;

GUASCONI, G ;

GERVY, P ;

LEGALL, I ;

SOULARUE, P ;

GRINAS, L ;

BOUGUELERET, L ;

BELLANNECHANTELOT, C ;

LACROIX, B ;

BARILLOT, E ;

GESNOUIN, P ;

POOK, S ;

VAYSSEIX, G ;

FRELAT, G ;

SCHMITZ, A ;

SAMBUCY, JL ;

BOSCH, A ;

ESTIVILL, X ;

WEISSENBACH, J ;

VIGNAL, A ;

RIETHMAN, H ;

COX, D ;

PATTERSON, D ;

GARDINER, K ;

HATTORI, M ;

SAKAKI, Y ;

ICHIKAWA, H ;

OHKI, M ;

LEPASLIER, D ;

HEILIG, R ;

ANTONARAKIS, S ;

COHEN, D .

NATURE, 1992, 359 (6394) :380-387

[7] A 1ST-GENERATION PHYSICAL MAP OF THE HUMAN GENOME [J].

COHEN, D ;

CHUMAKOV, I ;

WEISSENBACH, J .

NATURE, 1993, 366 (6456) :698-701

[8] THE MAMMALIAN PSEUDOAUTOSOMAL REGION [J].

ELLIS, N ;

GOODFELLOW, PN .

TRENDS IN GENETICS, 1989, 5 (12) :406-410

[9] THE PSEUDOAUTOSOMAL BOUNDARY IN MAN IS DEFINED BY AN ALU REPEAT SEQUENCE INSERTED ON THE Y-CHROMOSOME [J].

ELLIS, NA ;

GOODFELLOW, PJ ;

PYM, B ;

SMITH, M ;

PALMER, M ;

FRISCHAUF, AM ;

GOODFELLOW, PN .

NATURE, 1989, 337 (6202) :81-84

[10] THE HUMAN Y-CHROMOSOME - OVERLAPPING DNA CLONES SPANNING THE EUCHROMATIC REGION [J].

FOOTE, S ;

VOLLRATH, D ;

HILTON, A ;

PAGE, DC .

SCIENCE, 1992, 258 (5079) :60-66

← 1 2 →