DEXAMETHASONE INDUCES AN INCREASE IN INTRACELLULAR AND MEMBRANE-ASSOCIATED LIPOCORTIN-1 (ANNEXIN-1) IN RAT ASTROCYTE PRIMARY CULTURES

被引：17

作者：

MCLEOD, JD

BOLTON, C

机构：

[1] Department of Pharmacology, School of Pharmacy and Pharmacology, University of Bath, Claverton Down, BA2 7AY, Bath

来源：

CELLULAR AND MOLECULAR NEUROBIOLOGY | 1995年 / 15卷 / 02期

关键词：

ASTROCYTES; GLUCOCORTICOIDS; LIPOCORTIN-1(ANNEXIN 1);

D O I：

10.1007/BF02073328

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

1. The antiinflammatory actions of glucocorticoid steroids are thought to occur through induction of the protein lipocortin-1 (LC-1;annexin-1). The purpose of the current study was to investigate whether astrocytic LC-1 content was increased in the presence of a synthetic glucocorticoid, dexamethasone. 2. Steroid-induced changes in cellular levels of LC-1 in astrocytes were determined by electrotransfer and immunoblotting techniques. Separate cell fractions were investigated to study the influence of dexamethasone on astroglial LC-1 content, The effect of culture state an LC-1 expression was also examined. 3. Intracellular LC-1 content was found to decrease after initiation of culture, with a substantial rise in both cell proliferation and LC-1 expression occurring after the replenishment of medium containing steroid-free serum, A further increase in intracellular LC-1 occurred upon incubation with dexamethasone, The glucocorticoid-induced change in intracellular LC-1 was a time-dependent event and coincided with an increase in membrane-associated LC-1. 4. The findings in this study indicate that astrocytic LC-1 content is influenced by cell culture conditions and, in the presence of glucocorticoid steroids, the cellular localization of LC-1 is altered. This may indicate that LC-1 has functions at more than one cellular locality.

引用

页码：193 / 205

页数：13

共 43 条

[1] Ambrose M.P., Hunninghake G.W., Corticosteroids increase lipocortin 1 in BAL fluid from normal individuals and patients with lung disease, J. Appl. Physiol., 68, pp. 1668-1671, (1990)
[2] Ando Y., Imamura S., Owada M.K., Kannagi R., Calcium-induced intracellular cross-linking of lipocortin 1 by tissue transglutaminase in A431 cells, J. Biol. Chem., 226, pp. 1101-1108, (1991)
[3] Bienkowski M.J., Petro M.A., Robinson L.J., Inhibition of thromboxane A synthesis in U937 cells by glucocorticoids. Lack of evidence for lipocortin 1 as the second messenger, J. Biol. Chem., 264, pp. 6536-6544, (1989)
[4] Blackwell G.J., Carnuccio R., Di Rosa M., Flower R.J., Langham C.S.J., Parente L., Persico P., Russell-Smith N.C., Stone D., Glucocorticoids induce the formation and release of anti-inflammatory and anti-phospholipase proteins into the peritoneal cavity of the rat, Br. J. Pharmacol., 76, pp. 185-194, (1982)
[5] Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, pp. 248-254, (1976)
[6] Browning J.L., Ward M.P., Wallner B.P., Pepinsky R.B., Studies on the structural properties of lipocortin 1 and the regulation of its synthesis by steroids, Cytokines and Lipocortins in Inflammation and Differentation, pp. 27-45, (1990)
[7] Campos-Gonzalez R., Kanemitsu M., Boynton A.L., Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling, Cell Motil. Cytoskel., 15, pp. 34-40, (1990)
[8] Cirino G., Flower R.J., Human recombinant lipocortin 1 inhibits prostacyclin production by human umbilical artery in vitro, Prostaglandins, 34, pp. 59-62, (1987)
[9] Cole R., de Vellis J., Preparation of astrocyte and oligodendrocyte cultures from primary rat glial cultures, A Dissection and Tissue Culture Manual of the Nervous System, pp. 121-133, (1989)
[10] Croxtall J.D., Flower R.J., Lipocortin 1 mediates dexamethasone-induced growth arrest of the A549 lung adenocarcinoma cell line, Proc. Natl. Acad. Sci. USA, 89, pp. 3571-3575, (1992)

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