CLONING AND CHARACTERIZATION OF E2F-2, A NOVEL PROTEIN WITH THE BIOCHEMICAL-PROPERTIES OF TRANSCRIPTION FACTOR-E2F

被引：198

作者：

IVEYHOYLE, M ^{[1
]}

CONROY, R ^{[1
]}

HUBER, HE ^{[1
]}

GOODHART, PJ ^{[1
]}

OLIFF, A ^{[1
]}

HEIMBROOK, DC ^{[1
]}

机构：

[1] MERCK RES LABS,DEPT CANC RES,W POINT,PA 19486

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1993年 / 13卷 / 12期

关键词：

D O I：

10.1128/MCB.13.12.7802

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

E2F is a mammalian transcription factor that appears to play an important role in cell cycle regulation. While at least two proteins (E2F-1 and DP-1) with E2F-like activity have been cloned, studies from several laboratories suggest that additional homologs may exist. A novel protein with E2F-like properties, designated E2F-2, was cloned by screening a HeLa cDNA library with a DNA probe derived from the DNA binding domain of E2F-1 (K. Helin, J. A. Lees, M. Vidal, N. Dyson, E. Harlow, and A. Fattaey, Cell 70:337-350, 1992). E2F-2 exhibits overall 46% amino acid identity to E2F-1. Both the sequence and the function of the DNA and retinoblastoma gene product binding domains of E2F-1 are conserved in E2F-2. The DNA binding activity of E2F-2 is dramatically enhanced by complementation with particular sodium dodecyl sulfate-polyacrylamide gel electrophoresis-purified components of HeLa cell E2F, and anti-E2F-2 antibodies cross-react with components of purified HeLa cell E2F. These observations are consistent with a model in which E2F binds DNA as a heterodimer of two distinct proteins, and E2F-2 is functionally and immunologically related to one of these proteins.

引用

页码：7802 / 7812

页数：11

共 43 条

[1] ADENOVIRUS E1A PROTEINS CAN DISSOCIATE HETEROMERIC COMPLEXES INVOLVING THE E2F TRANSCRIPTION FACTOR - A NOVEL MECHANISM FOR E1A TRANSACTIVATION [J].