MEMBRANE INTERACTION AND CONFORMATIONAL PROPERTIES OF THE PUTATIVE FUSION PEPTIDE OF PH-30, A PROTEIN ACTIVE IN SPERM-EGG FUSION

被引:87
作者
MUGA, A
NEUGEBAUER, W
HIRAMA, T
SUREWICZ, WK
机构
[1] NATL RES COUNCIL CANADA, STEACIE INST MOLEC SCI, OTTAWA K1A 0R6, ON, CANADA
[2] NATL RES COUNCIL CANADA, INST BIOL SCI, OTTAWA K1A 0R6, ON, CANADA
[3] UNIV BASQUE COUNTRY, DEPT BIOCHEM, BILBAO, SPAIN
关键词
D O I
10.1021/bi00181a002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A peptide representing the putative fusion domain of PH-30, a sperm surface protein involved in sperm-egg fusion, was synthesized, and its interaction with model lipid membranes was characterized by biophysical methods. While the peptide binds to the vesicles composed of both neutral and acidic lipids, the apparent affinity is significantly higher for the latter lipid class. The intervesicular lipid mixing assay suggests that the synthetic peptide is able to induce fusion of large unilamellar vesicles. Circular dichroism and Fourier-transform infrared spectroscopy show that while in an aqueous buffer the peptide exists in an essentially unordered conformation, binding to the membranes results in a conformational transition to a beta-structure. These data indicate that the fragment identified on the alpha-subunit of PH-30 as a putative fusion peptide is indeed a good candidate for this role. However, in contrast to what has been proposed for some viral fusion peptides, the PH-30 fusion domain is highly unlikely to act as an insertional ''sided'' helix.
引用
收藏
页码:4444 / 4448
页数:5
相关论文
共 35 条
[1]   BEHAVIOR OF OXONOL DYES IN PHOSPHOLIPID DISPERSIONS [J].
BASHFORD, CL ;
CHANCE, B ;
SMITH, JC ;
YOSHIDA, T .
BIOPHYSICAL JOURNAL, 1979, 25 (01) :63-85
[2]   PROTEOLYTIC PROCESSING OF A PROTEIN INVOLVED IN SPERM EGG FUSION CORRELATES WITH ACQUISITION OF FERTILIZATION COMPETENCE [J].
BLOBEL, CP ;
MYLES, DG ;
PRIMAKOFF, P ;
WHITE, JM .
JOURNAL OF CELL BIOLOGY, 1990, 111 (01) :69-78
[3]   A POTENTIAL FUSION PEPTIDE AND AN INTEGRIN LIGAND DOMAIN IN A PROTEIN ACTIVE IN SPERM-EGG FUSION [J].
BLOBEL, CP ;
WOLFSBERG, TG ;
TURCK, CW ;
MYLES, DG ;
PRIMAKOFF, P ;
WHITE, JM .
NATURE, 1992, 356 (6366) :248-252
[4]   IDENTIFICATION OF THE FUSION PEPTIDE OF PRIMATE IMMUNODEFICIENCY VIRUSES [J].
BOSCH, ML ;
EARL, PL ;
FARGNOLI, K ;
PICCIAFUOCO, S ;
GIOMBINI, F ;
WONGSTAAL, F ;
FRANCHINI, G .
SCIENCE, 1989, 244 (4905) :694-697
[5]   ORIENTATION INTO THE LIPID BILAYER OF AN ASYMMETRIC AMPHIPATHIC HELICAL PEPTIDE LOCATED AT THE N-TERMINUS OF VIRAL FUSION PROTEINS [J].
BRASSEUR, R ;
VANDENBRANDEN, M ;
CORNET, B ;
BURNY, A ;
RUYSSCHAERT, JM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1029 (02) :267-273
[6]   CONFORMATIONS OF SIGNAL PEPTIDES INDUCED BY LIPIDS SUGGEST INITIAL STEPS IN PROTEIN EXPORT [J].
BRIGGS, MS ;
CORNELL, DG ;
DLUHY, RA ;
GIERASCH, LM .
SCIENCE, 1986, 233 (4760) :206-208
[7]   LIPID MIXING DURING MEMBRANE AGGREGATION AND FUSION - WHY FUSION ASSAYS DISAGREE [J].
DUZGUNES, N ;
ALLEN, TM ;
FEDOR, J ;
PAPAHADJOPOULOS, D .
BIOCHEMISTRY, 1987, 26 (25) :8435-8442
[8]   MEMBRANE DESTABILIZATION BY N-TERMINAL PEPTIDES OF VIRAL ENVELOPE PROTEINS [J].
DUZGUNES, N ;
SHAVNIN, SA .
JOURNAL OF MEMBRANE BIOLOGY, 1992, 128 (01) :71-80
[9]   PEPTIDE MODELS FOR THE MEMBRANE DESTABILIZING ACTIONS OF VIRAL FUSION PROTEINS [J].
EPAND, RM ;
CHEETHAM, JJ ;
EPAND, RF ;
YEAGLE, PL ;
RICHARDSON, CD ;
ROCKWELL, A ;
DEGRADO, WF .
BIOPOLYMERS, 1992, 32 (04) :309-314
[10]   SOLID-PHASE PEPTIDE-SYNTHESIS UTILIZING 9-FLUORENYLMETHOXYCARBONYL AMINO-ACIDS [J].
FIELDS, GB ;
NOBLE, RL .
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1990, 35 (03) :161-214