A SMALL METALLORIBOZYME WITH A 2-STEP MECHANISM

被引：201
作者：
PAN, T
UHLENBECK, OC
机构：
[1] Department of Chemistry and Biochemistry, University of Colorado, Boulder
来源：
NATURE | 1992年 / 358卷 / 6387期
关键词：
D O I：
10.1038/358560a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5' cleavage product terminates with a 3' phosphomonoester generated from a 2',3'-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA.
引用
收藏
页码：560 / 563
页数：4
相关论文
共 19 条
[1]  
Altman S., 1989, ADV ENZYMOL RAMB, V62, P1
[2]   LEAD-CATALYZED CLEAVAGE OF YEAST TRANSFER RNAPHE MUTANTS [J].
BEHLEN, LS ;
SAMPSON, JR ;
DIRENZO, AB ;
UHLENBECK, OC .
BIOCHEMISTRY, 1990, 29 (10) :2515-2523
[3]   PB(II)-CATALYSED CLEAVAGE OF THE SUGAR-PHOSPHATE BACKBONE OF YEAST TRANSFER RNAPHE - IMPLICATIONS FOR LEAD TOXICITY AND SELF-SPLICING RNA [J].
BROWN, RS ;
HINGERTY, BE ;
DEWAN, JC ;
KLUG, A .
NATURE, 1983, 303 (5917) :543-546
[4]   CRYSTALLOGRAPHIC AND BIOCHEMICAL INVESTIGATION OF THE LEAD(II)-CATALYZED HYDROLYSIS OF YEAST PHENYLALANINE TRANSFER-RNA [J].
BROWN, RS ;
DEWAN, JC ;
KLUG, A .
BIOCHEMISTRY, 1985, 24 (18) :4785-4801
[5]   BIOLOGICAL CATALYSIS BY RNA [J].
CECH, TR ;
BASS, BL .
ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :599-629
[6]   THE CHEMISTRY OF SELF-SPLICING RNA AND RNA ENZYMES [J].
CECH, TR .
SCIENCE, 1987, 236 (4808) :1532-1539
[7]  
CECH TR, 1990, REV BIOCH, V59, P5543
[8]   ROLE OF DIVALENT METAL-IONS IN THE HAMMERHEAD RNA CLEAVAGE REACTION [J].
DAHM, SC ;
UHLENBECK, OC .
BIOCHEMISTRY, 1991, 30 (39) :9464-9469
[9]   DEPOLYMERIZATION OF RIBONUCLEIC ACID BY PLUMBOUS ION [J].
FARKAS, WR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 155 (02) :401-&
[10]   SELF-CLEAVAGE OF VIRUSOID RNA IS PERFORMED BY THE PROPOSED 55-NUCLEOTIDE ACTIVE-SITE [J].
FORSTER, AC ;
SYMONS, RH .
CELL, 1987, 50 (01) :9-16
12