COAXIAL CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT FOR HIGHER-MOLECULAR-WEIGHT PEPTIDES - COMPARISON WITH STATIC FAST-ATOM-BOMBARDMENT AND ELECTROSPRAY IONIZATION

被引:11
作者
TOMER, KB
PERKINS, JR
PARKER, CE
DETERDING, LJ
机构
[1] Laboratory of Molecular Biophysics, National Institute for Environmental Health Sciences, North Carolina, 27709, Research Triangle Park
关键词
D O I
10.1002/bms.1200201207
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A comparison of coaxial continuous flow fast atom bombardment (FAB) with static FAB and with electrospray ionization (ESI) for the analysis of 'high'-mass peptides (M(r) = 3000-4000) is presented. Sensitivities of the peptides by coaxial continuous flow FAB is nearly an order of magnitude better than by static FAB. Single-scan spectra with good signal-to-noise can be obtained from as little as 200 fmol (by flow injection analysis). Detection limits by ESI mass spectrometry were found to be equivalent to 20 times higher than by coaxial continuous flow FAB on a per mole basis, but 4-20 times lower on a concentration basis, owing to the greater flow per unit time employed in the ESI mass spectrometric experiments.
引用
收藏
页码:783 / 788
页数:6
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