CREB - A CA2+-REGULATED TRANSCRIPTION FACTOR PHOSPHORYLATED BY CALMODULIN-DEPENDENT KINASES

被引：1394

作者：

SHENG, M ^{[1
]}

THOMPSON, MA ^{[1
]}

GREENBERG, ME ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

SCIENCE | 1991年 / 252卷 / 5011期

关键词：

D O I：

10.1126/science.1646483

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mechanism by which Ca2+ mediates gene induction in response to membrane depolarization was investigated. The adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB) was shown to function as a Ca2+-regulated transcription factor and as a substrate for depolarization-activated Ca2+-calmodulin-dependent protein kinases (CaM kinases) I and II. CREB residue Ser133 was the major site of phosphorylation by the CaM kinases in vitro and of phosphorylation after membrane depolarization in vivo. Mutation of Ser133 impaired the ability of CREB to respond to Ca2+. These results suggest that CaM kinases may transduce electrical signals to the nucleus and that CREB functions to integrate Ca2+ and cAMP signals.

引用

页码：1427 / 1430

页数：4

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