H-1-NMR STUDIES OF NATIVE AND FRAGMENTED SUBTILISIN CARLSBERG

被引：6

作者：

CONSONNI, R

MOLINARI, H

GRECO, F

ZANNONI, G

ZETTA, L

CARREA, G

RIVA, S

机构：

[1] CNR,INST CHIM MACROMOLEC,VIA AMPERE 56,I-20131 MILAN,ITALY

[2] CNR,IST CHIM ORMONI,I-20133 MILAN,ITALY

[3] UNIV MILAN,DIPARTIMENTO CHIM ORGAN & IND,I-20122 MILAN,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1119卷 / 01期

关键词：

NMR; SUBTILISIN CARLSBERG; PROTEINASE; AUTOLYSIS; SELECTIVE EXCITATION; SPIN ECHO;

D O I：

10.1016/0167-4838(92)90231-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR studies have been carried out on subtilisin Carlsberg in order to identify the sharp resonances observed in the proton spectra of the enzyme dissolved in aqueous solution. NMR spectra, obtained with the combination of spin-echo and selective excitation sequences, from both the native and inactivated protein, enabled us to assign sharp signals to subtilisin fragments derived from enzymatic autolysis (monitored by high-performance size-exclusion chromatography), rather than to mobile segments of the intact protein.

引用

页码：39 / 44

页数：6

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