USE OF RECOMBINANT DNA TECHNOLOGY TO PROGRAM EUKARYOTIC CELLS TO SYNTHESIZE RAT PROINSULIN - A RAPID EXPRESSION ASSAY FOR CLONED GENES

被引：61
作者：
LOMEDICO, PT
机构：
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES | 1982年 / 79卷 / 19期
关键词：
D O I：
10.1073/pnas.79.19.5798
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
引用
收藏
页码：5798 / 5802
页数：5
相关论文
共 36 条
[1]   METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES [J].
ALWINE, JC ;
KEMP, DJ ;
STARK, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5350-5354
[2]   SPLICED EARLY MESSENGER-RNAS OF SIMIAN VIRUS-40 [J].
BERK, AJ ;
SHARP, PA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (03) :1274-1278
[3]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[4]   A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].
BOYER, HW ;
ROULLAND.D .
JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&
[5]   SV40-DNA TRANSFECTION OF CELLS IN SUSPENSION - ANALYSIS OF THE EFFICIENCY OF TRANSCRIPTION AND TRANSLATION OF T-ANTIGEN [J].
CHU, G ;
SHARP, PA .
GENE, 1981, 13 (02) :197-202
[6]   SIMIAN VIRUS-40 MESSENGER-RNAS CONTAIN MULTIPLE 5'-TERMINI UPSTREAM AND DOWNSTREAM FROM A HOGNESS-GOLDBERG SEQUENCE - A SHIFT IN 5'-TERMINI DURING THE LYTIC CYCLE IS MEDIATED BY LARGE T-ANTIGEN [J].
GHOSH, PK ;
LEBOWITZ, P .
JOURNAL OF VIROLOGY, 1981, 40 (01) :224-240
[7]   RIBONUCLEIC-ACID ISOLATED BY CESIUM-CHLORIDE CENTRIFUGATION [J].
GLISIN, V ;
CRKVENJAKOV, R ;
BYUS, C .
BIOCHEMISTRY, 1974, 13 (12) :2633-2637
[8]   SV40-TRANSFORMED SIMIAN CELLS SUPPORT THE REPLICATION OF EARLY SV40 MUTANTS [J].
GLUZMAN, Y .
CELL, 1981, 23 (01) :175-182
[9]   NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].
GRAHAM, FL ;
VANDEREB, AJ .
VIROLOGY, 1973, 52 (02) :456-467
[10]   EXPRESSION OF SIMIAN VIRUS-40-RAT PREPROINSULIN RECOMBINANTS IN MONKEY KIDNEY-CELLS - USE OF PREPROINSULIN RNA PROCESSING SIGNALS [J].
GRUSS, P ;
KHOURY, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (01) :133-137
1234