A CONVENIENT, NONINVASIVE METHOD FOR MEASURING THE KINETICS OF PERMEATION OF DIMETHYL-SULFOXIDE INTO ISOLATED CORNEAS USING NMR-SPECTROSCOPY

被引：0

作者：

TAYLOR, MJ ^{[1
]}

BUSZA, AL ^{[1
]}

机构：

[1] ROYAL COLL SURG ENGLAND,HUNTERIAN INST,DEPT BIOPHYS,LONDON WC2A 3PN,ENGLAND

来源：

CRYO-LETTERS | 1992年 / 13卷 / 04期

关键词：

CORNEAS; CRYOPROTECTANT PERMEATION KINETICS; DIMETHYL SULFOXIDE; H-1 NMR SPECTROSCOPY;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Data for the kinetics of influx of cryoprotectants into tissues is essential for ensuring equilibration and for designing optimised procedures for cryopreservation. Proton nuclear magnetic resonance spectroscopy is used as a non invasive method for assessing the permeation of dimethyl sulphoxide (Me2SO) into isolated rabbit corneas at 0-degrees-C. Corneas reached 64% equilibration within 10 minutes and 82% after 2 hours. Permeation was significantly faster in de-epithelialised corneas.

引用

页码：273 / 282

页数：10

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