COMBINED USE OF RFLP AND PCR-ASO-TYPING FOR HLA-DR-DW-TYPING AND DQW-TYPING

被引：5
作者：
BIGNON, JD [1 ]
BIDWELL, JL [1 ]
机构：
[1] UNITED KINGDOM TRANSPLANT SERV, MOLEC GENET LAB, BRISTOL, ENGLAND
来源：
EUROPEAN JOURNAL OF IMMUNOGENETICS | 1991年 / 18卷 / 1-2期
关键词：
D O I：
10.1111/j.1744-313X.1991.tb00012.x
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization.
引用
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页码：125 / 138
页数：14
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