VOLATILE ANESTHETICS AND GLUTAMATE ACTIVATION OF N-METHYL-D-ASPARTATE RECEPTORS

Several studies have indicated important functional interactions between volatile anesthetics and the N-methyl-D-aspartate (NMDA) class of glutamate receptors. In the present study, we examined the effects of diethyl ether, chloroform, methoxyflurane, halothane, enflurane, and isoflurane on (1) glutamate activation of the NMDA receptor complex, including glycine reversal of anesthetic action, as revealed by [H-3] (5R,10S)-(+)methyl-10,11-dihydro-5H-dibbenzo[a,d]cyclohepten-5,10-imine, dizocilpine (MK-801) binding to the cation channel, and (2) [H-3]cis-4-(phosphonomethyl)piperidine-2-carboxylic acid (CGS 19755) binding to the glutamate recognition site of the NMDA receptor. In agreement with previous studies, glutamate increased the binding of 1 nM [H-3]MK-801, measured after a 1-hr incubation at 37 degrees, by up to several hundred fold. This stimulation was blocked by glutamate antagonists and potentiated by glycine with an EC(50) of approximate to 0.03 mu M. Glycine also had a direct stimulatory effect on [H-3]MK-801 binding at much higher concentrations (greater than or equal to 10 mu M). All of the anesthetics examined depressed glutamate stimulation of [H-3]MK-801 binding in a concentration-dependent manner with the following order of potency: halothane greater than or equal to enflurane > methoxyflurane > chloroform > diethyl ether. This inhibition of [H-3]MK-801 binding was observed at concentrations that are routinely attained in the cerebrospinal fluid during surgical anesthesia. Moreover, the inhibition was reversed rapidly following removal of the anesthetics from the assay medium. Inclusion of glycine in the incubation medium markedly attenuated anesthetic-induced inhibition of glutamate-sensitive [H-3]MK-801 binding with an EC(50) of between 0.1 and 1 mu M. Thus, this reversal by glycine correlated with its potentiating, as opposed to its direct stimulatory, effect on NMDA receptors. Anesthetic inhibition of [H-3]MK-801 binding could not be overcome by raising the glutamate concentration (i.e. the interaction did not appear to be competitive with respect to glutamate) unless glycine was present. Binding of [H-3]CGS 19755 to the glutamate recognition site was also inhibited by each of the anesthetics examined. However, with the exception of chloroform, all of the anesthetics were more potent inhibitors of glutamate-stimulated [H-3]MK-801 binding than they were of [H-3]CGS 19755 binding. [H-3]CGS 19755 binding saturation curves in the presence of halothane and enflurane indicated a decrease in the density of [H-3]-CGS 19755 binding sites with no change in binding affinity (i.e. the inhibition did not appear to be competitive). These findings support the idea that anesthetic drugs disrupt NMDA receptor transmission through multiple allosteric effects on the receptor-channel activation mechanism and the glutamate binding site.