TRANS SPLICING OF NEMATODE PRE-MESSENGER-RNA INVITRO

被引：84
作者：
HANNON, GJ
MARONEY, PA
DENKER, JA
NILSEN, TW
机构：
[1] Department of Molecular Biology, Microbiology Case Western Reserve University School of Medicine Cleveland
来源：
CELL | 1990年 / 61卷 / 07期
关键词：
D O I：
10.1016/0092-8674(90)90689-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5′ terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mRNA at an authentic trans splice acceptor site. SL addition occurs via a trans splicing reaction that proceeds through Y-branched intermediates. The branch-point is located at either of two adenosine residues located 18 and 19 nucleotides upstream of the splice acceptor site. © 1990.
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页码：1247 / 1255
页数：9
相关论文
共 31 条
[1]   PRESENCE OF THE CAENORHABDITIS-ELEGANS SPLICED LEADER ON DIFFERENT MESSENGER-RNAS AND IN DIFFERENT GENERA OF NEMATODES [J].
BEKTESH, S ;
VANDOREN, K ;
HIRSH, D .
GENES & DEVELOPMENT, 1988, 2 (10) :1277-1283
[2]   C-ELEGANS MESSENGER-RNAS ACQUIRE A SPLICED LEADER THROUGH A TRANS-SPLICING MECHANISM [J].
BEKTESH, SL ;
HIRSH, DI .
NUCLEIC ACIDS RESEARCH, 1988, 16 (12) :5692-5692
[3]   CIS AND TRANS MESSENGER-RNA SPLICING IN C-ELEGANS [J].
BLUMENTHAL, T ;
THOMAS, J .
TRENDS IN GENETICS, 1988, 4 (11) :305-308
[4]   DISCONTINUOUS TRANSCRIPTION AND ANTIGENIC VARIATION IN TRYPANOSOMES [J].
BORST, P .
ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :701-732
[5]   TRANS SPLICING INVOLVES A NOVEL FORM OF SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES [J].
BRUZIK, JP ;
VANDOREN, K ;
HIRSH, D ;
STEITZ, JA .
NATURE, 1988, 335 (6190) :559-562
[6]   ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].
DIGNAM, JD ;
LEBOVITZ, RM ;
ROEDER, RG .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489
[7]   MESSENGER-RNA SPLICING INVITRO - AN EXCISED INTERVENING SEQUENCE AND A POTENTIAL INTERMEDIATE [J].
GRABOWSKI, PJ ;
PADGETT, RA ;
SHARP, PA .
CELL, 1984, 37 (02) :415-427
[8]   ACCURATE PROCESSING OF HUMAN PRE-RRNA INVITRO [J].
HANNON, GJ ;
MARONEY, PA ;
BRANCH, A ;
BENENFIELD, BJ ;
ROBERTSON, HD ;
NILSEN, TW .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (10) :4422-4431
[9]   INTRON SPLICING - A CONSERVED INTERNAL SIGNAL IN INTRONS OF ANIMAL PRE-MESSENGER RNAS [J].
KELLER, EB ;
NOON, WA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (23) :7417-7420
[10]   TRANS SPLICING OF MESSENGER-RNA PRECURSORS INVITRO [J].
KONARSKA, MM ;
PADGETT, RA ;
SHARP, PA .
CELL, 1985, 42 (01) :165-171
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