Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids

被引:0
|
作者
Yulug, IG
Killary, AM
Sandhu, AK
Athwal, RS
Fox, M
Fisher, EMC
机构
[1] ST MARYS HOSP,IMPERIAL COLL,SCH MED,DEPT BIOCHEM & MOLEC GENET,LONDON W2 1PG,ENGLAND
[2] UNIV TEXAS,MD ANDERSON CANC CTR,DIV LAB MED,SECT HEMATOL PATHOL,HOUSTON,TX 77098
[3] TEMPLE UNIV,SCH MED,FELS INST CANC RES & MOL BIOL,PHILADELPHIA,PA 19140
[4] MRC,HUMAN BIOCHEM GENET UNIT,GALTON LAB,LONDON NW1 2HE,ENGLAND
关键词
D O I
10.1007/BF02257471
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To access a wide variety of expressed sequences from human chromosome 21 we have placed this chromosome into undifferentiated P19 mouse embryonic carcinoma cells. Cell lines resulting from these experiments have a range of morphologies and a wide variety of karyotypes. We have studied the retinoic acid response of five cell lines, compared to P19 cells, by observing three markers of retinoic acid induced P19 differentiation-cell morphology, RAR alpha and Wnt1 transcription. We see an 'early' retinoic acid response effect, however this response breaks down by the time the 'late' gene Wnt1 would be transcribed in P19 cells. A highly responsive cell line will be useful for cloning expressed sequences from human chromosome 21 which are produced by early genes in retinoic acid inducible pathway, such as those involved in neurogenesis.
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页码:357 / 365
页数:9
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