HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DIODE-ARRAY DETECTION OF PHOTOSYNTHETIC PIGMENTS

被引：7
作者：
ALMELA, L
FERNANDEZLOPEZ, JA
LOPEZROCA, JM
机构：
[1] Department of Agricultural Chemistry, University of Murcia, E-30100 Murcia, Campus of Espinardo
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 607卷 / 02期
关键词：
D O I：
10.1016/0021-9673(92)87078-M
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
High-performance liquid chromatography and photodiode-array detection were applied simultaneously for the separation and identification of the photosynthetic pigments of a plant extract. Using a photodiode-array detector permitted simultaneous recording of the chromatographic analysis at different wavelengths, the contour plots of the chromatograms and the spectra of the pigments separated by high-performance liquid chromatography. Employing these techniques, and on the basis of their spectroscopic characteristics, the following compounds were identified: beta-carotene, lutein, taraxanthin, violaxanthin, neoxanthin and chlorophylls a and b and their respective epimers, chlorophylls a' and b'. The rapidity, specificity, sensitivity and reproducibility of this technique make it particularly suitable for plant pigment analysis.
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页码：215 / 219
页数：5
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