Characterization and Genetic Analysis of Rumpled and Twisted Leaf Mutant (rtl1) in Rice

被引:1
作者
Fang Yun-xia [1 ]
Song Xiu-juan [1 ,2 ]
Peng You-lin [1 ,3 ]
Dong Guo-jun [1 ]
Guo Long-biao [1 ]
Zeng Da-li [1 ]
Zhang Guang-heng [1 ]
Yan Hong-lan [1 ]
Qian Qian [1 ]
机构
[1] China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
[2] Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310016, Zhejiang, Peoples R China
[3] Yangzhou Univ, Minist Educ, Key Lab Plant Funct Genom, Yangzhou 225009, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
rice; leaf type; genetic analysis; gene mapping; molecular marker; rumpled and twisted leaf mutant;
D O I
10.1016/S1672-6308(12)60001-8
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A rumpled and twisted leaf 1 (rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment, which was characterized as rumpled and twisted leaf at the seedling stage. The F-2 populations were constructed by crossing with indica cultivars TN1 and Zhefu 802, respectively. Genetic analysis demonstrated that the phenotype was controlled by a single recessive nuclear gene. The closely linked simple sequence repeat (SSR) marker RM1155 was obtained from bulked segregant analysis. Subsequently, sequence tagged site (STS) markers were developed using the published rice genome sequence. Finally, RTL1 was located between an STS marker T1591 and an SSR marker RM1359, at the distances of 0.48 cM and 0.96 cM, respectively. These results will facilitate the cloning of the target gene in further studies.
引用
收藏
页码:243 / 249
页数:7
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