SEPARATION OF MONO-PHASE, DI-PHASE, AND TRISTEARIN FROM AN INDUSTRIAL MIXTURE OF GLYCERIDES BY NORMAL-PHASE AND REVERSE-PHASE HPLC

被引：5
|
作者：
BAUZA, R
YSAMBERTT, F
MARQUEZ, N
DERODRIGUEZ, GO
LOPEZ, JL
机构：
[1] UNIV ZULIA,FAC EXPTL CIENCIAS,DEPT QUIM,APARTADO 526,MARACAIBO 4002,VENEZUELA
[2] COMPLEJO PETROQUIM ZULIA,INVEST & DESARROLLO CA,MARACAIBO 4002,VENEZUELA
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 1992年 / 27卷 / 05期
关键词：
D O I：
10.1080/01496399208018908
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This work reports on a quantitative and qualitative study of reverse and normal-phase HPLC to separate mono-, di-, and tristearin from an industrial mixture of glycerides. For normal-phase chromatography a CN-bonded packed column was used, while C8 and C18 columns were used for reverse-phase chromatography. Both types of chromatography were studied with UV and RI detection. The influence of some chromatographic factors, such as the bonded phase, mobile-phase composition, and flow rate, on the retention times, selectiveness, and effectiveness were studied.
引用
收藏
页码：645 / 661
页数：17
相关论文
共 50 条
  • [31] Normal-phase HPLC Separation of Possible Biosynthetic Intermediates of Pheophytin a and Chlorophyll a′
    Akimasa Nakamura
    Shuhei Tanaka
    Tadashi Watanabe
    Analytical Sciences, 2001, 17 : 509 - 513
  • [32] REVERSE-PHASE SEPARATION AND ELECTROCHEMICAL DETECTION OF NEUROPEPTIDES
    DAWSON, R
    STEVES, JP
    LORDEN, JF
    OPARIL, S
    PEPTIDES, 1985, 6 (06) : 1173 - 1178
  • [33] USE OF REVERSE-PHASE HPLC TO ISOLATE BIOACTIVITY FROM BSA
    KEENAN, J
    DOHERTY, G
    CLYNES, M
    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 1995, 14 (01) : 65 - 67
  • [34] A MODEL FOR RETENTION OF SOLUTES IN NORMAL-PHASE HPLC
    CAFFEY, K
    SNYDER, L
    SALOTTO, A
    WEISER, E
    FOSTER, J
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1985, 189 (APR-): : 7 - CHED
  • [35] MECHANISM AND PREDICTION OF RETENTION OF OLIGOMERS IN NORMAL-PHASE AND REVERSED-PHASE HPLC
    JANDERA, P
    CHROMATOGRAPHIA, 1988, 26 : 417 - 422
  • [36] HPLC/FTIR INTERFACE FOR NORMAL-PHASE AND REVERSED-PHASE ANALYTICAL COLUMNS
    KALASINSKY, VF
    WHITEHEAD, KG
    KENTON, RC
    SMITH, JAS
    KALASINSKY, KS
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1987, 25 (07) : 273 - 280
  • [37] Metal-organic framework MIL-100(Fe) as the stationary phase for both normal-phase and reverse-phase high performance liquid chromatography
    Fu, Yan-Yan
    Yang, Cheng-Xiong
    Yan, Xiu-Ping
    JOURNAL OF CHROMATOGRAPHY A, 2013, 1274 : 137 - 144
  • [38] Derivatization, separation and determination of steroids and triterpenoids by reverse-phase HPLC and UV detection
    Wang, SP
    Lai, JS
    Lin, ST
    JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 1997, 44 (04) : 395 - 399
  • [39] Application of statistical design for the optimization of amino acid separation by reverse-phase HPLC
    Gheshlaghi, R.
    Scharer, J. M.
    Moo-Young, M.
    Douglas, P. L.
    ANALYTICAL BIOCHEMISTRY, 2008, 383 (01) : 93 - 102
  • [40] SEPARATION AND ANALYSIS OF ORGANELLE-SPECIFIC TUBULIN SUBUNITS BY REVERSE-PHASE HPLC
    STEPHENS, RE
    JOURNAL OF CELL BIOLOGY, 1984, 99 (04) : A37 - A37
12345