A NOVEL MAGNETIC SILICA SUPPORT FOR USE IN CHROMATOGRAPHIC AND ENZYMATIC BIOPROCESSING

被引：32

作者：

GOETZ, V ^{[1
]}

REMAUD, M ^{[1
]}

GRAVES, DJ ^{[1
]}

机构：

[1] UNIV PENN,DEPT CHEM ENGN,PHILADELPHIA,PA 19104

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 07期

关键词：

CHROMATOGRAPHY; IMMOBILIZED ENZYME; MAGNETICALLY STABILIZED FLUIDIZED BED; SILICA;

D O I：

10.1002/bit.260370704

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A limited number of support matrices have so far been developed for use in magnetically stabilized fluidized bed (MSFB) applications. We have developed a versatile magnetic silica support which can be derivatized readily for both adsorption chromatography and enzyme immobilization by well-known techniques. A magnetic pellicular bead is prepared by electrostatically depositing alternating layers of colloidal silica and cationic polymer onto macroscopic nickel core particles. The polymer is then burned out and the silica partially sintered to yield a porous shell with 5-80 m2/g of surface area. This magnetic composite was tested as a support for immobilizing invertase. Sucrose was continuously converted to its component monosaccharides with nearly constant activity over the first 8 days and retention of 50% of initial activity after 25 days.

引用

页码：614 / 626

页数：13