PROTEIN-KINASE-C ACTIVATION AMPLIFIES PROSTAGLANDIN-F2-ALPHA-INDUCED PROSTAGLANDIN-E2 SYNTHESIS IN OSTEOBLAST-LIKE CELLS

被引：24
作者：
TOKUDA, H
OISO, Y
KOZAWA, O
机构：
[1] AICHI PREFECTURAL COLONY,INST DEV RES,DEPT BIOCHEM,KASUGAI,AICHI 48003,JAPAN
[2] NAGOYA UNIV,SCH MED,DEPT INTERNAL MED 1,NAGOYA,AICHI 466,JAPAN
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 1992年 / 48卷 / 03期
关键词：
PROTEIN KINASE-C; PROSTAGLANDIN; ARACHIDONIC ACID; PHOSPHOLIPASE-A; OSTEOBLAST;
D O I：
10.1002/jcb.240480306
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In cloned osteoblast-like cells, MC3T3-E1, prostaglandin F2-alpha (PGF2-alpha) stimulated arachidonic acid (AA) release in a dose-dependent manner in the range between 1 nM and 10-mu-M. 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C (PKC) activator, which by itself had little effect on AA release, markedly amplified the release of AA stimulated by PGF2-alpha in a dose-dependent manner. 4 alpha-phorbol 12,13-didecanoate, a phorbol ester which is inactive for PKC, showed little effect on the PGF2-alpha-induced AA release. 1-oleoyl-2-acetylglycerol (OAG), a specific activator for PKC, mimicked TPA by enhancement of the AA release induced by PGF2-alpha. H-7, a PKC inhibitor, markedly suppressed the effect of OAG on PGF2-alpha-induced AA release. Quinacrine, a phospholipase A2 inhibitor, showed partial inhibitory effect on PGF2-alpha-induced AA release, while it suppressed the amplification by OAG of PGF2-alpha-induced AA release almost to the control level. Furthermore, TPA enhanced the AA release induced by melittin, known as a phospholipase A2 activator. On the other hand, TPA inhibited the formation of inositol trisphosphate stimulated by PGF2-alpha. Under the same condition, PGF2-alpha indeed stimulated prostaglandin E2 (PGE2) synthesis and TPA markedly amplified the PGF2-alpha-induced PGE2 synthesis as well as AA release. These results indicate that the activation of PKC amplifies PGF2-alpha-induced both AA release and PGE2 synthesis through the potentiation of phospholipase A2 activity in osteoblast-like cells.
引用
收藏
页码：262 / 268
页数：7
相关论文
共 50 条
  • [1] PROSTAGLANDIN-F2-ALPHA ACTIVATES PHOSPHOLIPASE-D INDEPENDENTLY FROM ACTIVATION OF PROTEIN-KINASE-C IN OSTEOBLAST-LIKE CELLS
    KOZAWA, O
    SUZUKI, A
    KOTOYORI, J
    TOKUDA, H
    WATANABE, Y
    ITO, Y
    OISO, Y
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 55 (03) : 373 - 379
  • [2] AUTOREGULATION OF PROSTAGLANDIN-E(2)-INDUCED CA2+ INFLUX IN OSTEOBLAST-LIKE CELLS - INHIBITION BY SELF-INDUCED ACTIVATION OF PROTEIN-KINASE-C
    TOKUDA, H
    MIWA, M
    OISO, Y
    KOZAWA, O
    CELLULAR SIGNALLING, 1992, 4 (03) : 261 - 266
  • [3] REGULATION OF ASTROCYTE PROLIFERATION BY PROSTAGLANDIN-E2 AND THE ALPHA SUBTYPE OF PROTEIN-KINASE-C
    SAWADA, M
    SUZUMURA, A
    OHNO, K
    MARUNOUCHI, T
    BRAIN RESEARCH, 1993, 613 (01) : 67 - 73
  • [4] PROSTAGLANDIN-E2 IS A POTENTIAL MEDIATOR OF EXTRACELLULAR ATP ACTION IN OSTEOBLAST-LIKE CELLS
    SUZUKI, A
    KOTOYORI, J
    OISO, Y
    KOZAWA, O
    CELL ADHESION AND COMMUNICATION, 1993, 1 (02) : 113 - 118
  • [5] INVOLVEMENT OF PROTEIN-KINASE-A AND PROTEIN-KINASE-C IN THE PRODUCTION OF INTERLEUKIN-1(ALPHA)-INDUCED PROSTAGLANDIN E(2) FROM MOUSE OSTEOBLAST-LIKE CELL-LINE, MC3T3-E1
    IITAKA, M
    KITAHAMA, S
    ISHII, J
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1994, 1221 (01): : 78 - 82
  • [6] EVIDENCE OF A ROLE FOR PROTEIN-KINASE-C IN EPIDERMAL GROWTH FACTOR-INDUCED PROSTAGLANDIN-E2 SYNTHESIS IN AMNION CELLS
    KNISS, DA
    MERSHON, J
    SU, HC
    SONEK, J
    FERTEL, RH
    WAXMAN, M
    IAMS, JD
    GABBE, SG
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1990, 163 (06) : 1883 - 1890
  • [7] EFFECTS OF VITAMIN-D3 ON SIGNALING BY PROSTAGLANDIN-E2 IN OSTEOBLAST-LIKE CELLS
    TOKUDA, H
    KOTOYORI, J
    SUZUKI, A
    OISO, Y
    KOZAWA, O
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, 52 (02) : 220 - 226
  • [8] Prostaglandin F-2 alpha stimulates interleukin-6 synthesis via activation of PKC in osteoblast-like cells
    Kozawa, O
    Suzuki, A
    Tokuda, H
    Uematsu, T
    AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1997, 272 (02): : E208 - E211
  • [9] PROSTAGLANDIN-INDUCED CHANGES IN CALCIUM-UPTAKE AND CAMP PRODUCTION IN OSTEOBLAST-LIKE CELLS - ROLE OF PROTEIN-KINASE-C
    HAGELBRADWAY, S
    TATAKIS, DN
    DZIAK, R
    CALCIFIED TISSUE INTERNATIONAL, 1991, 48 (04) : 272 - 277
  • [10] EFFECT OF PROSTAGLANDIN-F(2-ALPHA) ON CA2+ INFLUX IN OSTEOBLAST-LIKE CELLS - FUNCTION OF TYROSINE KINASE
    SUZUKI, A
    KOZAWA, O
    SAITO, H
    OISO, Y
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 54 (04) : 487 - 493
12345