SIMPLIFIED MEASUREMENT OF SERUM ALKALINE-PHOSPHATASE UTILIZING ELECTROCHEMICAL DETECTION OF 4-AMINOPHENOL

被引：22
|
作者：
CHRISTIE, IM [1 ]
TRELOAR, PH [1 ]
KOOCHAKI, ZB [1 ]
VADGAMA, PM [1 ]
SMITH, GN [1 ]
机构：
[1] UNIV MANCHESTER,DEPT CHEM,MANCHESTER M13 9PL,LANCS,ENGLAND
来源：
ANALYTICA CHIMICA ACTA | 1992年 / 257卷 / 01期
关键词：
AMPEROMETRY; ALKALINE PHOSPHATASE; AMINOPHENOL; SERUM;
D O I：
10.1016/0003-2670(92)80145-W
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Alkaline phosphatase is determined amperometrically using a simple two-electrode system; results are presented as a kinetic increase in electroactive product. Of the substrates investigated, 4-aminophenyl phosphate gives rise to the optimum signal against background interference in serum. The results are correlated with a routine spectrophotometric assay. The final method is unaffected by interference or fouling problems, with electrodes covered by either a non-selective dialysis membrane or a selective cellulose acetate membrane. Diffusion through both membranes is sufficiently fast for following the kinetic response.
引用
收藏
页码：21 / 28
页数：8
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