SIMPLIFIED MEASUREMENT OF SERUM ALKALINE-PHOSPHATASE UTILIZING ELECTROCHEMICAL DETECTION OF 4-AMINOPHENOL

被引：22

作者：

CHRISTIE, IM ^{[1
]}

TRELOAR, PH ^{[1
]}

KOOCHAKI, ZB ^{[1
]}

VADGAMA, PM ^{[1
]}

SMITH, GN ^{[1
]}

机构：

[1] UNIV MANCHESTER,DEPT CHEM,MANCHESTER M13 9PL,LANCS,ENGLAND

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 257卷 / 01期

关键词：

AMPEROMETRY; ALKALINE PHOSPHATASE; AMINOPHENOL; SERUM;

D O I：

10.1016/0003-2670(92)80145-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Alkaline phosphatase is determined amperometrically using a simple two-electrode system; results are presented as a kinetic increase in electroactive product. Of the substrates investigated, 4-aminophenyl phosphate gives rise to the optimum signal against background interference in serum. The results are correlated with a routine spectrophotometric assay. The final method is unaffected by interference or fouling problems, with electrodes covered by either a non-selective dialysis membrane or a selective cellulose acetate membrane. Diffusion through both membranes is sufficiently fast for following the kinetic response.

引用

页码：21 / 28

页数：8

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