7H8/6, A MULTICOPY DNA PROBE FOR DISTINGUISHING ISOLATES OF PLASMODIUM-FALCIPARUM

被引：44
作者：
LIMPAIBOON, T
SHIRLEY, MW
KEMP, DJ
SAUL, A
机构：
[1] QUEENSLAND INST MED RES,BRAMSTON TERRACE,HERSTON,QLD 4006,AUSTRALIA
[2] ROYAL MELBOURNE HOSP,WALTER & ELIZA HALL INST MED RES,PARKVILLE,VIC 3050,AUSTRALIA
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 47卷 / 02期
基金：
英国医学研究理事会;
关键词：
PLASMODIUM-FALCIPARUM; DNA FINGERPRINTING; POLYMORPHIC PROBE; CHROMOSOME MAPPING;
D O I：
10.1016/0166-6851(91)90179-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method using DNA fingerprinting to distinguish Plasmodium falciparum isolates has been developed using a clone, 7H8/6, isolated by screening a genomic expression library of the Malayan Camp strain with a monoclonal antibody. Unlike other P. falciparum DNA probes that contain internally repetitive sequences, this probe contains a single full-length open reading frame and lacks any repetitive sequences. Chromosome mapping located the hybridizing sequences to most chromosomes. 7H8/6 sequences are located at sites near the subtelomeric regions, although they are further from the telomeres than the subtelomeric repetitive sequences rep20. The probe gave multiple polymorphic bands on Southern blots of genomic DNA allowing all unrelated isolates tested to be readily distinguished. Hybridization patterns of parent and progeny from the genetic cross of two P. falciparum clones showed that most bands were inherited independently and no new bands were generated during the cross. This probe is useful for distinguishing isolates of P. falciparum and also for analyzing relationships between closely related isolates.
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页码：197 / 206
页数：10
相关论文
共 32 条
[11]   EVIDENCE FOR A SWITCHING MECHANISM IN THE INVASION OF ERYTHROCYTES BY PLASMODIUM-FALCIPARUM [J].
DOLAN, SA ;
MILLER, LH ;
WELLEMS, TE .
JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (02) :618-624
[12]   AMPLIFICATION OF THE MULTIDRUG RESISTANCE GENE IN SOME CHLOROQUINE-RESISTANT ISOLATES OF P-FALCIPARUM [J].
FOOTE, SJ ;
THOMPSON, JK ;
COWMAN, AF ;
KEMP, DJ .
CELL, 1989, 57 (06) :921-930
[13]   CHROMOSOMES OF MALARIA PARASITES [J].
FOOTE, SJ ;
KEMP, DJ .
TRENDS IN GENETICS, 1989, 5 (10) :337-342
[14]   THE ESTABLISHMENT OF GENOMIC DNA LIBRARIES FOR THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM AND IDENTIFICATION OF INDIVIDUAL CLONES BY HYBRIDIZATION [J].
GOMAN, M ;
LANGSLEY, G ;
HYDE, JE ;
YANKOVSKY, NK ;
ZOLG, JW ;
SCAIFE, JG .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1982, 5 (06) :391-400
[15]   PLASMODIUM-FALCIPARUM - ANALYSIS OF CHROMOSOMES SEPARATED BY CONTOUR-CLAMPED HOMOGENOUS ELECTRIC-FIELDS [J].
GU, HM ;
INSELBURG, JW ;
BZIK, DJ ;
LI, WB .
EXPERIMENTAL PARASITOLOGY, 1990, 71 (02) :189-198
[16]   ANTIGENS OF THE ERYTHROCYTIC STAGES OF THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM DETECTED BY MONOCLONAL-ANTIBODIES [J].
HALL, R ;
MCBRIDE, J ;
MORGAN, G ;
TAIT, A ;
ZOLG, JW ;
WALLIKER, D ;
SCAIFE, J .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1983, 7 (03) :247-265
[17]  
LIMPAIBOON T, 1990, Southeast Asian Journal of Tropical Medicine and Public Health, V21, P388
[18]   ANTIGENIC DIVERSITY IN THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM [J].
MCBRIDE, JS ;
WALLIKER, D ;
MORGAN, G .
SCIENCE, 1982, 217 (4556) :254-257
[19]   CHARACTERIZATION OF A REPETITIVE DNA-SEQUENCE FROM THE MALARIA PARASITE, PLASMODIUM-FALCIPARUM [J].
OQUENDO, P ;
GOMAN, M ;
MACKAY, M ;
LANGSLEY, G ;
WALLIKER, D ;
SCAIFE, J .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1986, 18 (01) :89-101
[20]   CHROMOSOME SIZE POLYMORPHISM IN PLASMODIUM-FALCIPARUM CAN INVOLVE DELETIONS OF THE SUBTELOMERIC PPFREP20 SEQUENCE [J].
PATARAPOTIKUL, J ;
LANGSLEY, G .
NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4331-4340
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