SOMATIC EMBRYOGENESIS FROM PEA EMBRYOS AND SHOOT APICES

被引：49
作者：
KYSELY, W
JACOBSEN, HJ
机构：
[1] UNIV BONN,INST GENET,KIRSCHALLEE 1,W-5300 BONN 1,GERMANY
[2] WOLFGANG BOCK PFLANZENEXPORT KG,W-2800 BREMEN BORGFELD,GERMANY
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 20卷 / 01期
关键词：
genotype; Pisum sativum; plant growth regulators; plant regeneration; somatic embryogenesis; tissue culture;
D O I：
10.1007/BF00034751
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Conditions were defined for plant regeneration via somatic embryogenesis in pea, using explants from immature zygotic embryos or from shoot apices. For the induction of somatic embryos, an auxin (picloram or 2,4-dichlorophenoxyacetic acid) was required. Embryogenic callus originated from embryonic axis tissue of immature embryos and from the axillary-bud region and the plumula of shoot apices. A clear effect of embryo size on somatic embryogenesis was shown. There were differences in frequency of somatic embryogenesis among the five genotypes used in the study. Additions of BA to auxin-containing medium reduced embryo production. Histological examinations confirmed the embryogenic nature of the immature embryo cultures and revealed that somatic embryos originated from the meristematic areas near the callus surface. © 1990 Kluwer Academic Publishers.
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页码：7 / 14
页数：8
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