THE MACROPHAGE AND B-CELL SPECIFIC TRANSCRIPTION FACTOR PU.1 IS RELATED TO THE ETS ONCOGENE

被引：949
作者：
KLEMSZ, MJ
MCKERCHER, SR
CELADA, A
VANBEVEREN, C
MAKI, RA
机构：
[1] Cancer Research Center La Jolla Cancer Research Foundation, La Jolla, CA 92037
来源：
CELL | 1990年 / 61卷 / 01期
关键词：
D O I：
10.1016/0092-8674(90)90219-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated a cDNA clone, PU.1, that codes for a new tissue-specific DNA binding protein. Analysis of the binding site by methylation interference and DNA-ase I protection revealed that the PU.1 protein recognized a purine-rich sequence, 5′-GAGGAA-3′ (PU box). The PU.1 protein was shown to be a transcriptional activator that is expressed in macrophages and B cells. cDNA constructions used to generate proteins lacking portions of either the amino- or carboxy-terminal ends of the PU.1 protein placed the DNA binding domain in the highly basic carboxy-terminal domain of the protein. The amino acid sequence in the binding domain of PU.1 has considerable identity with proteins belonging to the ets oncogene family. © 1990.
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页码：113 / 124
页数：12
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