DIRECTED MUTAGENESIS IN CANDIDA-ALBICANS - ONE-STEP GENE DISRUPTION TO ISOLATE URA3 MUTANTS

被引：148

作者：

KELLY, R ^{[1
]}

MILLER, SM ^{[1
]}

KURTZ, MB ^{[1
]}

KIRSCH, DR ^{[1
]}

机构：

[1] SQUIBB INST MED RES,DEPT BLDG STUDIES,PRINCETON,NJ 08540

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1987年 / 7卷 / 01期

关键词：

D O I：

10.1128/MCB.7.1.199

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：199 / 207

页数：9

共 35 条

[1] EVIDENCE FOR TRANSCRIPTIONAL REGULATION OF OROTIDINE-5'-PHOSPHATE DECARBOXYLASE IN YEAST BY HYBRIDIZATION OF MESSENGER-RNA TO THE YEAST STRUCTURAL GENE CLONED IN ESCHERICHIA-COLI [J].

BACH, ML ;

LACROUTE, F ;

BOTSTEIN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (01) :386-390

[2] A POSITIVE SELECTION FOR MUTANTS LACKING OROTIDINE-5'-PHOSPHATE DECARBOXYLASE ACTIVITY IN YEAST - 5-FLUORO-OROTIC ACID RESISTANCE [J].

BOEKE, JD ;

LACROUTE, F ;

FINK, GR .

MOLECULAR & GENERAL GENETICS, 1984, 197 (02) :345-346

[3] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[4] STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].

BOTSTEIN, D ;

FALCO, SC ;

STEWART, SE ;

BRENNAN, M ;

SCHERER, S ;

STINCHCOMB, DT ;

STRUHL, K ;

DAVIS, RW .

GENE, 1979, 8 (01) :17-24

[5] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[6]

BROACH JR, 1979, GENE, V8, P121, DOI 10.1016/0378-1119(79)90012-X

[7]

CASE ME, 1980, P NATL ACAD SCI US, V77, P5259

[8] ISOLATION OF THE CANDIDA-ALBICANS GENE FOR OROTIDINE-5'-PHOSPHATE DECARBOXYLASE BY COMPLEMENTATION OF S-CEREVISIAE URA3 AND ESCHERICHIA-COLI PYRF MUTATIONS [J].

GILLUM, AM ;

TSAY, EYH ;

KIRSCH, DR .

MOLECULAR & GENERAL GENETICS, 1984, 198 (01) :179-182

[9] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].

HOLMES, DS ;

QUIGLEY, M .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) :193-197

[10]

HUGHES J, 1983, MORTAL WEEKLY REP SU, V32, P1

← 1 2 3 4 →