RELATIONSHIPS BETWEEN INTERMEDIATE TCR CELLS AND NK1.1(+) T-CELLS IN VARIOUS IMMUNE ORGANS - NK1.1(+) T-CELLS ARE PRESENT WITHIN A POPULATION OF INTERMEDIATE TCR CELLS

Experiments to date have revealed a population of T cells that carry intermediate (int) levels of TCR (or CD3) and express IL-2R beta-chain (IL-2R beta) in mouse liver. Such int TCR cells also reside in other immune organs, although in low numbers. On the other hand, NK1.1(+) T cells with int TCR do reside in the thymus and other peripheral organs. To determine the relationship of two types of cells, we characterized int CD3 cells and NK1.1(+) T cells throughout the organs in terms of the phenotype, V beta repertoire, and morphology. Although both IL-2R beta(+) T cells and NK1.1(+) T cells are classified as int CD3 cells, NK1.1(+) T cells are present within int CD3 cells. The majority of int CD3 cells in the liver and thymus were NK1.1(+), whereas the minority of such cells in the spleen, lymph nodes, and bone marrow were NK1.1(+). Among int CD3 cells, double-negative (DN) CD4(-)8(-) cells and/or CD4(+) were abundant in NK1.1(+) subset, whereas CD8(+) cells were generally abundant in NK1.1(-) subset. Self-reactive V beta(+) clones estimated by the M1s system were distributed to both NK1.1(+) and NK1.1(-) subsets. High CD3 cells in the thymus and other organs contained neither DN cells nor forbidden clones. Int CD3 cells had the morphology of granular or agranular lymphocytes carrying perforin. Among int CD3 cells, NK1.1(+) subset had a higher level of perforin-positive cells than NK1.1(-) subset. These results clearly demonstrate the relationship between int TCR cells and NK1.1(+) T cells in various organs.