BIOLOGICAL-MATERIALS STUDIED WITH DYNAMIC FORCE MICROSCOPY

被引：37

作者：

ANSELMETTI, D

DREIER, M

LUTHI, R

RICHMOND, T

MEYER, E

FROMMER, J

GUNTHERODT, HJ

机构：

来源：

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B | 1994年 / 12卷 / 03期

关键词：

D O I：

10.1116/1.587272

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Biological materials such as hexagonal packed intermediate (HPI) layers, DNA, tobacco mosaic virus and collagen deposited on various substrates with noncontact dynamic force microscopy under ambient conditions were investigated. This method is highly suited for the investigation of soft organic matter where a minimized interaction between tip and sample is needed for nondestructive and reliable operation. Hence, additional anchoring of the biological specimens was no longer found to be crucial. The vertical and lateral resolution limits of this gentle method were determined to be <0.1 nm and 1-2 nm, respectively, allowing very stable and high resolution results on all investigated systems. By taking approach curves and monitoring the dynamic properties of the cantilever (resonance frequency and Q value) during the experiment, the interaction mechanism between tip and sample was found to-be dominated by attractive van der Waals interaction and capillary forces. Furthermore, initial results from a HPI layer imaged with noncontact dynamic force microscopy in a water environment are presented.

引用

页码：1500 / 1503

页数：4

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