KINETICS OF GERANYL ACETATE SYNTHESIS BY LIPASE-CATALYZED TRANSESTERIFICATION IN NORMAL-HEXANE

被引：133

作者：

CHULALAKSANANUKUL, W ^{[1
]}

CONDORET, JS ^{[1
]}

COMBES, D ^{[1
]}

机构：

[1] INST NATL SCI APPL,DEPT GENIE BIOCHIM & ALIMENTAIRE,CNRS,UA 544,AVE RANGUEIL,F-31077 TOULOUSE,FRANCE

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1992年 / 14卷 / 04期

关键词：

ENZYMATIC TRANSESTERIFICATION; GERANIOL; LIPASE; ORGANIC SOLVENT;

D O I：

10.1016/0141-0229(92)90154-G

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The synthesis of geranyl acetate by transesterification catalysed by immobilized lipase of Mucor miehei was studied. To obtain the highest yield for geranyl acetate synthesis, the effect of ester chain length on the rate of reaction was investigated. Propyl acetate was found to be the best substrate for geranyl acetate synthesis. The kinetics of reaction are suggested to agree with a ping-pong Bi Bi mechanism in which inhibition by excess of geraniol has been identified. The values of all apparent kinetic parameters were computed. No evidence of any significant external diffusional limitation that could account for these values has been detected. In order to define the optimal conditions of the reaction, optimization of water content through the distribution ratio of water between solvent and support was examined.

引用

页码：293 / 298

页数：6

共 18 条

[1]

ARMSTRONG DW, 1989, FLAVOR CHEM TRENDS D, P105

[2] KINETIC-STUDY OF ESTERIFICATION BY IMMOBILIZED LIPASE IN N-HEXANE [J].