INACTIVATION OF CONTRACTION AS A DETERMINANT OF LENGTH-ACTIVE TENSION RELATION IN HEART-MUSCLE OF CAT

In isometrically contracting isolated cat papillary muscles, the mechanism determining the reduction in active tension (P) at lengths (L) below the optimal length (Lmax) for maximal tension development (Pmax) was studied. Increasing [Ca2+] from 1.25-5 mM, paired electrical stimulation and caffeine augmented Pmax by 77.61 and 23%, respectively, as well as the tension developed at any given length on the ascending limb of the length-active tension relation. To see whether below Lmax the degree of activation is dependent on length, these length-active tension relations were expressed as P/Pmax relative to L/Lmax where Pmax and Lmax in each inotropic state was 100%. These normalized length-active tension relations were not altered by different [Ca2+]o, but shifted upward and to the left by caffeine and paired stimulation. The effects of elevating [Ca2+]o on the length-active tension relation suggests that restoring forces and fixed internal loads are not an important factor in decreasing active tension at short lengths. The effects of caffeine and paired stimulation support the view that in cardiac muscle, inactivation of contraction is a major factor determing the fall in active tension at decreasing length.