A RAPID AND SENSITIVE INVITRO ASSAY FOR THE ACTIVITY OF BACILLUS-THURINGIENSIS DELTA-ENDOTOXINS

被引：8

作者：

PARENTI, P ^{[1
]}

VILLA, M ^{[1
]}

TASCA, M ^{[1
]}

BELGIOJOSO, P ^{[1
]}

HANOZET, GM ^{[1
]}

GIORDANA, B ^{[1
]}

机构：

[1] UNIV MILAN, DEPT BIOL, I-20133 MILAN, ITALY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 104卷 / 02期

关键词：

D O I：

10.1016/0305-0491(93)90382-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Activated 6-endotoxins from Bacillus thuringiensis strains toxic to lepidopteran larvae inhibit K+-dependent accumulation of amino acids into brush border membrane vesicles (BBMV) from the midgut of the susceptible species Bombyx mori. 2. The activated toxins interfere with the K+-dependent uptake of histidine into BBMV only if they are effectively active in 3. To calculate IC50 values (the toxin concentration which determines 50%, of the effect), dose response curves were performed for each toxin. The values obtained correlate well with the LD50 determined by bioassay. 4. This amino acid inhibition test could represent a rapid (36 hr, compared to 3 4 days for bioassay) and sensitive method for the screening of larvicidal activity of known or new recombinant delta-endotoxins,

引用

页码：375 / 379

页数：5

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