STEROID SULFATES IN HUMAN BILE

被引：40

作者：

LAATIKAINEN, T

PELTOKALLIO, P

VIHKO, R

机构：

[1] Department of Medical Chemistry, Second Department of Surgery, University of Helsinki

来源：

STEROIDS | 1968年 / 12卷 / 03期

关键词：

D O I：

10.1016/0039-128X(68)90031-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steroid monosulfate and disulfate fractions were obtained from human bile by chromatography on Sephadex LH-20. The conjugates in these fractions had the mobility of steroid mono-and disulfates, respectively, in liquid - liquid partition chromatography on Celite, thin-layer chromatography and high-voltage paper electrophoresis. After solvolysis, the steroids were fractionated on silicic acid and analyzed by gas-liquid chromatography and gas chromatography - mass spectrometry. Androsterone, dehydroepiandrosterone, pregnenolone, 5α-pregnane-3α, 20α-diol and 5β-pregnane-3α, 20α-diol were found in the monosulfate fraction and 5a-androstane-3a, 17a-diol, 5α-androstane-3α, 17β-diol, androst-5-ene-3β, 17α-diol, androst-5-ene-3β, 17(3-diol, 5α-pregnane-3α, 20α-diol, and pregnane-3α, 20α-diol and pregn-5-ene-3(3,20a-diol in the disulfate fraction of bile steroids. © 1968.

引用

页码：407 / +

页数：1

共 38 条

[31] CHROMATOGRAPHY OF CONJUGATED STEROIDS ON LIPOPHILIC SEPHADEX [J].