IDENTIFICATION OF ENTEROPATHOGENIC ESCHERICHIA-COLI BY PCR-BASED DETECTION OF THE BUNDLE-FORMING PILUS GENE

被引：225
作者：
GUNZBURG, ST
TORNIEPORTH, NG
RILEY, LW
机构：
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 05期
关键词：
D O I：
10.1128/JCM.33.5.1375-1377.1995
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.
引用
收藏
页码：1375 / 1377
页数：3
相关论文
共 17 条
  • [1] PLASMID-MEDIATED ADHESION IN ENTEROPATHOGENIC ESCHERICHIA-COLI
    BALDINI, MM
    KAPER, JB
    LEVINE, MM
    CANDY, DCA
    MOON, HW
    [J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1983, 2 (03) : 534 - 538
  • [2] ENTEROPATHOGENIC ESCHERICHIA-COLI
    DONNENBERG, MS
    KAPER, JB
    [J]. INFECTION AND IMMUNITY, 1992, 60 (10) : 3953 - 3961
  • [3] A PLASMID-ENCODED TYPE-IV FIMBRIAL GENE OF ENTEROPATHOGENIC ESCHERICHIA-COLI ASSOCIATED WITH LOCALIZED ADHERENCE
    DONNENBERG, MS
    GIRON, JA
    NATARO, JP
    KAPER, JB
    [J]. MOLECULAR MICROBIOLOGY, 1992, 6 (22) : 3427 - 3437
  • [4] DISTRIBUTION OF THE BUNDLE-FORMING PILUS STRUCTURAL GENE (BFPA) AMONG ENTEROPATHOGENIC ESCHERICHIA-COLI
    GIRON, JA
    DONNENBERG, MS
    MARTIN, WC
    JARVIS, KG
    KAPER, JB
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (04) : 1037 - 1041
  • [5] AN INDUCIBLE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI
    GIRON, JA
    HO, ASY
    SCHOOLNIK, GK
    [J]. SCIENCE, 1991, 254 (5032) : 710 - 713
  • [6] GOMES TAT, 1991, J INFECT DIS, V164, P331, DOI 10.1093/infdis/164.2.331
  • [7] SINGLE PRIMER PAIR FOR AMPLIFYING SEGMENTS OF DISTINCT SHIGA-LIKE-TOXIN GENES BY POLYMERASE CHAIN-REACTION
    KARCH, H
    MEYER, T
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (12) : 2751 - 2757
  • [8] SCREENING FOR ENTEROPATHOGENIC ESCHERICHIA-COLI IN INFANTS WITH DIARRHEA BY THE FLUORESCENT-ACTIN STAINING TEST
    KNUTTON, S
    PHILLIPS, AD
    SMITH, HR
    GROSS, RJ
    SHAW, R
    WATSON, P
    PRICE, E
    [J]. INFECTION AND IMMUNITY, 1991, 59 (01) : 365 - 371
  • [9] THE DIARRHEAL RESPONSE OF HUMANS TO SOME CLASSIC SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA-COLI IS DEPENDENT ON A PLASMID ENCODING AN ENTEROADHESIVENESS FACTOR
    LEVINE, MM
    NATARO, JP
    KARCH, H
    BALDINI, MM
    KAPER, JB
    BLACK, RE
    CLEMENTS, ML
    OBRIEN, AD
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) : 550 - 559
  • [10] ESCHERICHIA-COLI THAT CAUSE DIARRHEA - ENTEROTOXIGENIC, ENTEROPATHOGENIC, ENTEROINVASIVE, ENTEROHEMORRHAGIC, AND ENTEROADHERENT
    LEVINE, MM
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (03) : 377 - 389
12