IDENTIFICATION OF ENTEROPATHOGENIC ESCHERICHIA-COLI BY PCR-BASED DETECTION OF THE BUNDLE-FORMING PILUS GENE

被引：226

作者：

GUNZBURG, ST

TORNIEPORTH, NG

RILEY, LW

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 05期

关键词：

D O I：

10.1128/JCM.33.5.1375-1377.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.

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页码：1375 / 1377

页数：3

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