IDENTIFICATION OF ENTEROPATHOGENIC ESCHERICHIA-COLI BY PCR-BASED DETECTION OF THE BUNDLE-FORMING PILUS GENE

被引：225
|
作者：
GUNZBURG, ST
TORNIEPORTH, NG
RILEY, LW
机构：
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 05期
关键词：
D O I：
10.1128/JCM.33.5.1375-1377.1995
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.
引用
收藏
页码：1375 / 1377
页数：3
相关论文
共 50 条
  • [1] AN INDUCIBLE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI
    GIRON, JA
    HO, ASY
    SCHOOLNIK, GK
    SCIENCE, 1991, 254 (5032) : 710 - 713
  • [2] Enteropathogenic Escherichia coli: Identification of a gene cluster coding for bundle-forming pilus morphogenesis
    Sohel, I
    Puente, JL
    Ramer, SW
    Bieber, D
    Wu, CY
    Schoolnik, GK
    JOURNAL OF BACTERIOLOGY, 1996, 178 (09) : 2613 - 2628
  • [3] DISTRIBUTION OF THE BUNDLE-FORMING PILUS STRUCTURAL GENE (BFPA) AMONG ENTEROPATHOGENIC ESCHERICHIA-COLI
    GIRON, JA
    DONNENBERG, MS
    MARTIN, WC
    JARVIS, KG
    KAPER, JB
    JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (04): : 1037 - 1041
  • [4] Structure of the bundle-forming pilus from enteropathogenic Escherichia coli
    Ramboarina, S
    Fernandes, PJ
    Daniell, S
    Islam, S
    Simpson, P
    Frankel, G
    Booy, F
    Donnenberg, MS
    Matthews, S
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (48) : 40252 - 40260
  • [5] Bundle-forming pilus retraction enhances enteropathogenic Escherichia coli infectivity
    Zahavi, Eitan E.
    Lieberman, Joshua A.
    Donnenberg, Michael S.
    Nitzan, Mor
    Baruch, Kobi
    Rosenshine, Ilan
    Turner, Jerrold R.
    Melamed-Book, Naomi
    Feinstein, Naomi
    Zlotkin-Rivkin, Efrat
    Aroeti, Benjamin
    MOLECULAR BIOLOGY OF THE CELL, 2011, 22 (14) : 2436 - 2447
  • [6] TRANSCRIPTIONAL REGULATION OF THE BUNDLE-FORMING PILIN GENE OF ENTEROPATHOGENIC ESCHERICHIA-COLI
    PUENTE, JL
    BIEBER, D
    MURRAY, WJ
    SOBEL, I
    SCHOOLNIK, GK
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 50 - 50
  • [7] Expression of the bundle-forming pilus by enteropathogenic Escherichia coli strains of heterologous serotypes
    Gismero-Ordoñez, J
    Dall'Agnol, M
    Trabulsi, LR
    Girón, JA
    JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) : 2291 - 2296
  • [8] The inner membrane subassembly of the enteropathogenic Escherichia coli bundle-forming pilus machine
    Crowther, LJ
    Anantha, RP
    Donnenberg, MS
    MOLECULAR MICROBIOLOGY, 2004, 52 (01) : 67 - 79
  • [9] Enteropathogenic Escherichia coli virulence factor bundle-forming pilus has a binding specificity for phosphatidylethanolamine
    Khursigara, C
    Abul-Milh, M
    Lau, B
    Girón, JA
    Lingwood, CA
    Foster, DEB
    INFECTION AND IMMUNITY, 2001, 69 (11) : 6573 - 6579
  • [10] Differentiation of typical and atypical enteropathogenic Escherichia coli using colony immunoblot for detection of bundle-forming pilus expression
    Nara, J. M.
    Cianciarullo, A. M.
    Culler, H. F.
    Bueris, V.
    Horton, D. S. P. Q.
    Menezes, M. A.
    Franzolin, M. R.
    Elias, W. P.
    Piazza, R. M. F.
    JOURNAL OF APPLIED MICROBIOLOGY, 2010, 109 (01) : 35 - 43
12345