QUANTITATIVE-ANALYSIS OF PHOSPHOROTHIOATE OLIGONUCLEOTIDES IN BIOLOGICAL-FLUIDS USING DIRECT-INJECTION FAST ANION-EXCHANGE CHROMATOGRAPHY AND CAPILLARY GEL-ELECTROPHORESIS

被引：58

作者：

BOURQUE, AJ ^{[1
]}

COHEN, AS ^{[1
]}

机构：

[1] HYBRIDON,WORCESTER,MA 01605

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 662卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00207-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of antisense phosphorothioate DNA (SODN) in human plasma via direct injection using anion-exchange high-performance liquid chromatography (AE-HPLC) is presented. The method relies on the ability to selectively extract phosphorothioate DNA from undigested serum, plasma and urine on anion-exchange resins. The automated HPLC method can analyze a sample every 5 min with a limit of detection of 50 ng/ml (ppb). The DNA was collected, desalted and analyzed by capillary gel electrophoresis. Due to the high resolving power of this technique, a qualitative assessment of enzymatic degradation of the antisense oligonucleotide can be made.

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页码：343 / 349

页数：7

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