PARTIAL-PURIFICATION AND PROPERTIES OF EXTRACELLULAR CHITINASE PRODUCED BY ACREMONIUM-OBCLAVATUM, AN ANTAGONIST TO THE GROUNDNUT RUST, PUCCINIA-ARACHIDIS

被引：20

作者：

GUNARATNA, KR ^{[1
]}

BALASUBRAMANIAN, R ^{[1
]}

机构：

[1] UNIV MADRAS, CTR ADV STUDY BOT, GUINDY CAMPUS, MADRAS 600025, TAMIL NADU, INDIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1994年 / 10卷 / 03期

关键词：

ACREMONIUM-OBCLAVATUM; CHITINASE; PUCCINIA-ARACHIDIS; UREDOSPORES;

D O I：

10.1007/BF00414876

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An extracellular chitinase of Acremonium obclavatum was partially purified. It had an M(r) of 45 kDa on SDS-PAGE, and was optimally active at pH 3 to 4 and 50-degrees-C. Hg and Mn (10 mm) inhibited activity. The chitinase hydrolysed colloidal chitin more rapidly than crude chitin or isolated A. obclavatum cell walls. The partially-purified enzyme inhibited uredospore germination and germ-tube growth of Puccinia arachidis.

引用

页码：342 / 345

页数：4

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