NOVEL DATA ON INTERACTIONS OF ELONGATION FACTOR-TS

被引：12

作者：

BUBUNENKO, MG ^{[1
]}

KIREEVA, ML ^{[1
]}

GUDKOV, AT ^{[1
]}

机构：

[1] ACAD SCI USSR, INST PROT RES, PUSHCHINO 142292, USSR

来源：

BIOCHIMIE | 1992年 / 74卷 / 05期

关键词：

ELONGATION FACTOR-TS; INTERACTION WITH EF-TU-AA-TRANSFER RNA COMPLEX; INTERACTION WITH RIBOSOME;

D O I：

10.1016/0300-9084(92)90081-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interactions of EF-Ts with EF-Tu at all steps of the elongation cycle were studied by limited trypsinolysis, gel-filtration, analytical centrifugation and fluorescence polarization techniques. It is shown that EF-Ts does not dissociate from EF-Tu after GDP to GTP exchange, but remains bound to the Aa-tRNA.EF-Tu.GTP complex up to GTP hydrolysis stage on the ribosome. The possible role of these interactions is discussed.

引用

页码：419 / 425

页数：7

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