ADJUSTMENT OF RESOLUTION AND ANALYSIS TIME IN CAPILLARY ZONE ELECTROPHORESIS BY VARYING THE PH OF THE BUFFER

被引：43

作者：

KENNDLER, E

FRIEDL, W

机构：

[1] Institute for Analytical Chemistry, University of Vienna, A-1090 Vienna

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 608卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)87119-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An expression for resolution in capillary zone electrophoresis is derived which is a function of the pH of the buffer. It is based on the conventional definitions of resolution, but takes into account the dependence of the efficiency term on the effective mobilities, or the charge numbers, of the separands (monovalent ions). The different shapes of the R vs. pH curves, as derived from the resolution equation, are discussed and found to agree with the experimental results. An approach for the selection of that single pH where a given resolution can be achieved within the shortest time of analysis is introduced.

引用

页码：161 / 170

页数：10

共 14 条

[1] DETERMINATION OF ABSOLUTE MOBILITIES, PK VALUES AND SEPARATION NUMBERS BY CAPILLARY ZONE ELECTROPHORESIS - EFFECTIVE MOBILITY AS A PARAMETER FOR SCREENING [J].