ACTIVATION OF P34CDC2 KINASE BY CYCLIN IS NEGATIVELY REGULATED BY CYCLIC AMP-DEPENDENT PROTEIN-KINASE IN XENOPUS-OOCYTES

被引:43
作者
RIME, H
HACCARD, O
OZON, R
机构
[1] Laboratoire de Physiologie de la Reproduction, UA-CNRS, INRA 1449, Université P. et M. Curie. 4, Place Jussieu
关键词
D O I
10.1016/0012-1606(92)90217-5
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Microinjection of a bacterially expressed stable Δ90 sea urchin cyclin B into Xenopus prophase oocytes, in absence or presence of cycloheximide, provokes the activation of histone H1 kinase and the tyrosine dephosphorylation of p34cdc2 Unexpectedly, when prophase oocytes are submitted to a treatment known to elevate the intracellular cAMP level (3-isobutyl-1-methylxanthine and cholera toxin), Δ90 cyclin has no effect and the oocytes remain blocked in prophase. This inhibition is reverted by the microinjection of the inhibitor of cAMP-dependent protein kinase. When Δ90 cyclin is microinjected into oocytes depleted of endogenous cyclins (cycloheximide-treated metaphase I) and in the presence of a high intracellular concentration of cAMP, p34cdc2 kinase is tyrosine rephosphorylated. Altogether, our results indicate that in Xenopus oocyte, cAMP-dependent protein kinase (A-kinase) controls the formation of the cyclin B/p34cdc2 complex which remains inactive and tyrosine phosphorylated. © 1992.
引用
收藏
页码:105 / 110
页数:6
相关论文
共 40 条
[1]   REGULATION OF MPF ACTIVITY INVITRO [J].
CYERT, MS ;
KIRSCHNER, MW .
CELL, 1988, 53 (02) :185-195
[2]   THE CDC25 PROTEIN CONTAINS AN INTRINSIC PHOSPHATASE-ACTIVITY [J].
DUNPHY, WG ;
KUMAGAI, A .
CELL, 1991, 67 (01) :189-196
[3]   FISSION YEAST P13 BLOCKS MITOTIC ACTIVATION AND TYROSINE DEPHOSPHORYLATION OF THE XENOPUS CDC2 PROTEIN-KINASE [J].
DUNPHY, WG ;
NEWPORT, JW .
CELL, 1989, 58 (01) :181-191
[4]   FISSION YEAST P107WEE1 MITOTIC INHIBITOR IS A TYROSINE SERINE KINASE [J].
FEATHERSTONE, C ;
RUSSELL, P .
NATURE, 1991, 349 (6312) :808-811
[5]   CYCLIN IS A COMPONENT OF MATURATION-PROMOTING FACTOR FROM XENOPUS [J].
GAUTIER, J ;
MINSHULL, J ;
LOHKA, M ;
GLOTZER, M ;
HUNT, T ;
MALLER, JL .
CELL, 1990, 60 (03) :487-494
[6]   CDC25 IS A SPECIFIC TYROSINE PHOSPHATASE THAT DIRECTLY ACTIVATES P34CDC2 [J].
GAUTIER, J ;
SOLOMON, MJ ;
BOOHER, RN ;
BAZAN, JF ;
KIRSCHNER, MW .
CELL, 1991, 67 (01) :197-211
[7]   CYCLIN-B IN XENOPUS OOCYTES - IMPLICATIONS FOR THE MECHANISM OF PRE-MPF ACTIVATION [J].
GAUTIER, J ;
MALLER, JL .
EMBO JOURNAL, 1991, 10 (01) :177-182
[8]   CELL-CYCLE DYNAMICS OF AN M-PHASE-SPECIFIC CYTOPLASMIC FACTOR IN XENOPUS-LAEVIS OOCYTES AND EGGS [J].
GERHART, J ;
WU, M ;
KIRSCHNER, M .
JOURNAL OF CELL BIOLOGY, 1984, 98 (04) :1247-1255
[9]   INVITRO EFFECTS ON MICROTUBULE DYNAMICS OF PURIFIED XENOPUS M-PHASE-ACTIVATED MAP KINASE [J].
GOTOH, Y ;
NISHIDA, E ;
MATSUDA, S ;
SHIINA, N ;
KOSAKO, H ;
SHIOKAWA, K ;
AKIYAMA, T ;
OHTA, K ;
SAKAI, H .
NATURE, 1991, 349 (6306) :251-254
[10]   XENOPUS M-PHASE MAP KINASE - ISOLATION OF ITS CDNA AND ACTIVATION BY MPF [J].
GOTOH, Y ;
MORIYAMA, K ;
MATSUDA, S ;
OKUMURA, E ;
KISHIMOTO, T ;
KAWASAKI, H ;
SUZUKI, K ;
YAHARA, I ;
SAKAI, H ;
NISHIDA, E .
EMBO JOURNAL, 1991, 10 (09) :2661-2668