IMMUNOCHARACTERIZATION OF DELTA-ISOENZYMES AND ZETA-ISOENZYMES OF PROTEIN-KINASE-C IN RAT RENAL MESANGIAL CELLS

被引：72

作者：

HUWILER, A

FABBRO, D

STABEL, S

PFEILSCHIFTER, J

机构：

[1] UNIV BASEL,BIOCTR,DEPT PHARMACOL,KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND

[2] CIBA GEIGY AG,RES DEPT,DIV PHARMACEUT,CH-4002 BASEL,SWITZERLAND

[3] MAX PLANCK GESELL,MAX DELBRUCK LAB,W-5000 COLOGNE 30,GERMANY

来源：

FEBS LETTERS | 1992年 / 300卷 / 03期

关键词：

PROTEIN KINASE-C; ISOENZYME; MESANGIAL CELL;

D O I：

10.1016/0014-5793(92)80858-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The isoforms of protein kinase C (PKC) present in rat mesangial cells were identified by immunoblot analysis with antibody raised against isotype-specific peptides. In addition to the previously observed alpha- and epsilon-subspecies, mesangial cells also express the delta-and zeta-isoenzymes of PKC. On exposure to phorbol 12,13-dibutyrate (PDB) a complete depletion of PKC-delta is observed within 8 h. Removal of PDB results in a recovery of PKC-delta. In contrast, PKC-zeta is unaffected by addition or removal of PDB.

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页码：259 / 262

页数：4

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