GLUCOSE-PRODUCTION FROM MALTOHEXAOSE AS A SUBSTRATE BY THE BACTERIAL ALPHA-AMYLASE-CATALYZED REACTION - ITS TIME COURSES OBTAINED BY A THEORETICAL AND AN EXPERIMENTAL APPROACHES

被引：4

作者：

IWATA, K ^{[1
]}

OHNISHI, M ^{[1
]}

HIROMI, K ^{[1
]}

机构：

[1] KYOTO UNIV,COLL AGR,DEPT FOOD SCI & TECHNOL,SAKYO WARD,KYOTO 606,JAPAN

来源：

STARCH-STARKE | 1990年 / 42卷 / 04期

关键词：

D O I：

10.1002/star.19900420406

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Based on the subsite structure of an α‐amylase from Bacillus subtilis, production of glucose from a substrate maltohexaose in the amylase‐catalyzed reaction was theoretically predicted as a function of time. The theoretical time course was found consistent with the experimental results, which were obtained by the determination of a product glucose with a commercial assay‐kit. Hence, glucose, product from a substrate maltohexaose, will be a useful probe for the amylase‐catalyzed reaction. Copyright © 1990 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：147 / 151

页数：5

共 7 条

[1] SUBSITE AFFINITIES OF BACTERIAL LIQUEFYING ALPHA-AMYLASE EVALUATED FROM RATE PARAMETERS OF LINEAR SUBSTRATES [J].

IWASA, S ;

AOSHIMA, H ;

HIROMI, K ;

HATANO, H .

JOURNAL OF BIOCHEMISTRY, 1974, 75 (05) :969-978

[2]

OHNISHI M, IN PRESS STARCH

[3]

OHNISHI M, 1971, J BIOCH, V69, P5621

[4]

SUGANUMA T, 1980, J BIOCHEM-TOKYO, V88, P131

[5]

SUGANUMA T, 1989, AMYLASE, P263

[6] SUBSITE MAPPING OF ENZYMES - STUDIES ON BACILLUS-SUBTILIS AMYLASE [J].

THOMA, JA ;

BROTHERS, C ;

SPRADLIN, J .

BIOCHEMISTRY, 1970, 9 (08) :1768-&

[7]

THOMA JA, 1971, J BIOL CHEM, V246, P181

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